Effects Of Acyl-CoA Oxidase On The Reproduction Of Nilaparvata Lugens (St(?)l) Induced By Triazophos

The brown planthopper(BPH), Nilaparvata lugens (Stal) (Homoptera: Delphacidae) is a serious pest of rice crops with long-distance migratory characteristics. The resurgence of BPH involves hormesis, compensation, physiological and molecular mechanisms of stimulated reproduction induced by pesticide and so on. Here, we study the changes of lipid metabolism and reproduction influenced by triazophos(TZP) through analyzing proteins of fat body in BPH females and RNA interference(RNAi).1. Lipid metabolism analysis in the fat body of BPH exposed to TZPThe lipid metabolism of insects is important for growth, reproduction, and fat body is the metabolism center of BPH. We study the lipid metabolism exposed to TZP through proteomic analysis.91 diff proteins related to lipid metabolism are detected, accounting for 18.1% in all diff proteins annotated to specific pathways. The most important diff proteins include pancreatic triglyceride lipase, glutamine synthetase, acetyl-cholinesterase, acyl-CoA oxidase and so on. Meanwhile,19 lipid metabolism pathways are concentrated (accounting for 8.5%), including the digestion and absorption of fat and other lipid metabolic pathways such as fatty acid metabolism. The most important pathway include biosynthesis of unsaturated fatty acids, glycerolipid metabolism, fatty acid metabolis, fatty acid elongation, glycerophospholipid metabolism, steroid hormone biosynthesis, inositol phosphate metabolism and so on. Thus, the lipid metabolism in the fat body of BPH has active changes after exposed to TZP.2.Influences on the reproduction of BPH females after interfering acyl-coenzyme A oxidase (ACO)We analyzed diff proteins of differently treated BPHs in this study, which showed TZP increased the expression of ACO. In the following RNAi experiment, we found that the reproduction of BPH decreased significantly when fed with artificial diet containing dsACO.The number of eggs laid of TZP+dsACO decreased 30%and 32%compared with TZP(from 530 to 366) and TZP+dsGFP treatments respectively. The oviposition period of TZP+dsACO decreased 24% and 21% compared with TZP and TZP+dsGFP treatments respectively. The longevity of TZP+dsACO decreased 20% and 26% compared with TZP and TZP+dsGFP treatments respectively. Contents of crude fat and protein are also significantly reduced in the body of BPH females. The contents of crude fat of TZP+dsACO decreased 42%compared with TZP (from 5.3mg to 3.1mg). The contents of fatty acid also had different variations. Suppressing ACO decreased the contents of oleic acid, palmitic acid, linoleic acid, stearic acid ester, and myristoleic acid in the fat body of BPH females. In addition, the contents of protein of TZP+dsACO decreased 11.8% compared with TZP (from 194ug to 3.lug). So, ACO is one of the genes showed a TZP-induced stimulation of BPH reproduction.