Effect Of Bacillus Coagulans On Intestinal Mucosal Barrier Structure And Functions Of Broilers With Pathogenic Bacteria

This study was performed to determine the effects of dietary Bacillus coagulans supplementation on intestinal mucosal barrier structure and Functions of Broilers with Pathogenic bacteria.Experment 1:The study was conducted to investigate the effects of dietary inclusion Bacillus coagulans on intestinal inflammation of broiler chickens, under a Salmonella Enteritis disease challenge. A total of 312 male broiler chicks were randomly assigned to 4 experimental treatments, each replicated 6 times, with 13 birds per replicate. The 4 treatment groups were an uninfected control an infected control and the level supplemented with Bacillus coagulans at 0 and 400 mg/kg of feed using a 2×2 factorial completely randomized design. Oral inoculation of the chanllenged group with Salmonella Enteritis occurred on d 9 to d 11. The rest of the birds (unchallenged group) received 1 mL of nutrient broth. The results showed thatBacillus coagulans showed a tendency to gain more BW in birds under the disease challenge. Bacillus coagulans supplementation can depress intestinal inflammation and serum oxidative damage and raise the antibacterial gene expression, increase immune function of intestine from bacterial infection. In conclusion, dietary B.coagulans supplementation could promote intestinal mucosa development and increase immune function, could be used as a prophylactic measurements for controling chicken Salmonella enteritis infection.Experment 2:The study was conducted to investigate the effects of dietary inclusion Bacillus coagulans on intestinal inflammation of broiler chickens, under a Clostridium perfringens disease challenge. A total of 312 male broiler chicks were randomly assigned to 4 experimental treatments, each replicated 6 times, with 13 birds per replicate. The 4 treatment groups were an uninfected control, an infected control and the level supplemented with Bacillus coagulans at 0 and 400 mg/kg of feed using a 2×2 factorial completely randomized design. Oral inoculation of the challenged group with Eimeria spp. occurred on d 13, followed by 3 consecutive inoculations of Clostridium perfringens from d 19 through 21. The rest of the birds (unchallenged group) received 1 mL of nutrient broth. The results showed that Bacillus coagulans can improve growth performance of broiler. Bacillus coagulans supplementation can depress intestinal inflammation and serum oxidative damage and raise the antibacterial gene expression, increase immune function of intestine from bacterial infection, In conclusion, dietary B.coagulans supplementation could promote intestinal mucosa development and increase immune function, could be used as a prophylactic measurements for controling chicken Salmonella enteritis infection.In conclusion, dietary supplementation could protect intestine barrier function from damage by intestinal pathogens via attenuating serum oxidative injury, Improving the antibacterial gene expression enhancing intestinal mucosal immune function. Therefore, Bacillus coagulans could be used as a prophylactic agent for control poultry entericpathogenic bacteria infection.