LncRNA Expression Signatures In Response To Enterotoxigenic Escherichia Coli Infection In Mice

Background:Enterotoxigenic Escherichia coli. is a global major pathogen causing severe watery diarrhea in young animals, causing huge losses to the livestock industry. ETEC is also an serious pathogen in children and travelers’ diarrhea in developing countries, and become an important public health threat to human safety. Adhesion and enterotoxins as the two main types of ETEC virulence factors often as the advantages of enterotoxigenic Escherichia coli vaccine antigens. However for infectious disease, the interaction mechanism of the host cell with pathogenic bacteria is significant. Recently, a large numbers of Lnc RNAs were found in eukaryotic cells, and the specific expressions of these lnc RNAs were related to cancer and many other diseases. Long non-coding RNA (LncRNA) is a class of nc RNAs with the length more than 200nt, which have no ORF encoding proteins. Initially, the non-coding transcripts were thought to be "transcriptional noise", which didn’t play any role in cell development and metabolic processes. However, LncRNA expression profile in intestinal infectious disease injury and repair is still unclear.Objectives:Building and evaluating the diarrhea model infected by ETEC; Screening the lnc RNA differentially expressed of ntestinal infectious disease infected by ETEC.Methods: Diarrhea model was prepared in mice via infected by ETEC, the evaluated of the model was using histopathology and cytokines. Total RNA was extracted from intestin of mice. After quantile normalization of the raw data, differentially expressed LncRNA and mRNA with statistical significance between the three groups were identified. Results: (1) After infected by ETEC, mice exhibited clinical symptoms of diarrhea, and the loss of weight. (2) Pathological slice observation results show that experimengt group infected by ETEC intestine tissue and mucosa are damaged seriously and microvilli has been fall off. whereas the control mice intestinal mucosa were in good condition, microvilli form completely. (3) Infected by ETEC,the cytokines of intestine tissue (IL-1β、IL-2、IL-3、IL-4、IL-5、IL-6, IL-10、 IL-23、 GM-CSF) was higher than the control group and there was statistically significant difference (P<0.05). Ratio of experiment group Thl/Th2 was lower than that of the control group. (4) Compared with control group,the LncRNA/mRNA expression profile in intestine tissue infected by ETEC showed statistical significance (p<0.05). We screened out 9 differentially expressed LncRNA disordered for more than 1.5 times, including 2 up regulated,and 7 down regulated; 26 differentially expressed mRNA disordered for more than 2 times,including 15 up regulated, and 11 down regulated.Conclusions:(1) After infected by ETEC, mice exhibited clinical symptoms of diarrhea, and the loss of weight, intestine tissue and mucosa are damaged seriously.(2)Infected by ETEC,the Th1、Th17 cytokines of intestine tissue were differential expression,and the balance of Thl、Th2 was destroyed.The inflammatory response has been happened.Therefore, the infection of ETEC is related to the happen of inflammatory response.(3) Compared with control group,Lnc RNA in intestine tissue infected by ETEC show differential expression.(4) LncRNA ENSMUST00000122226 may take part in the development process of intestinal infectious disease infected by ETEC...