Sequence Analysis Of Two Sweet PotatoVirus(SPFMV And SPCSV) In Guangxi And Preparation Of A Antiserum Of SPCSV

Viral disease is one of the main diseases of sweet potato in Guangxi province. Sweet potato samples with viral diseases symptoms like were collected from Nanning, Chongzuo, Beihai, Yulin of Guangxi. and then sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV) were detected by RT-PCR method. The results showed that SPFMV and SPCSV existwidespreadly in Guangxi. When sweet potato was single infected by SPFMV or SPCSV, sweet potato appeared mild symptoms of viral disease, if sweet potato were co-infected by both SPFMV and SPCSV, the symptoms showed more severe likedwarfleaf shrinking and so on.Primers were designed for amplification the CP gene of SPFMV and SPCSV, then the CP gene of 18 SPFMV isolates and 14 SPCSV isolates from Guangxi were cloned and sequenced. The CP gene nucleotide sequence of SPFMV Guangxi isolate was 945 bp, coded 315 amino acids; the CP gene nucleotide sequence of SPCSVGuangxi isolates was 774 bp, coded 257 amino acids.The results of CP gene sequence analysis of SPFMV and SPCSV showed that:three strains were found from 18 SPFMV Guangxi isolates, including O strain, RC strain and EA strain, C strain was not found in Guangxi, O strain of SPFMV Guangxi isolates was the advantage strain; There was only WA strain in SPCSV Guangxi isolates, no EA strain.The CP gene of SPCSV were amplified by RT-PCR, then cloned into pET30a(+) vector and transformed into BL21(DE3)pLysS, expressed by IPTG and the SPCSV CP protein was obtained as expectations. The protein were purified by recovering purification. Then immuned rabbit to get the antiserum of SPCSV CP. Antiserum weredetected by ELISA and Western Blot, the results showed that titer of the antiserum of SPCSV CP was 1:64000. SPCSV CP antiserum can be used to monitor SPCSV infection in sweet potato production.