Effects Of Sodium Gluconate On Gut Microflora、growth Development And Fatty Acid Synthesis Of AA~+ Broilers

When the antibiotics are banned to add in feed, there become forcus to look for a green, non-toxic,and low-cost feed addition to alternative to antibiotics. Sodium gluconate that a new feed addition, with stable, low cost, safe, reliable, has also been widespread attention in recentl years. Therefore, this article aims to study the effects of sodium gluconate on gut microflora, growth and fatty acid synthesis of broilers.The article is mainly divided into two experiment. Experiment 1: Two hundred and forty 1-day-old male AA + broilers that have similar weight were randomly divided into A, B, C, D four treatment groups with six replicates of 10 broilers. The control group that group A was fed the basal diet without sodium gluconate addition. For treatment group, sodium gluconate was added in feed at 0.10%, 0.25%,0.40% of basal diet. The experiment aims to study the effects of sodium gluconate on gut microflara and intestinal structure of broilers. Experiment 2: Two hundred and forty 1-day-old male AA + broilers that have similar weight were randomly divided into A, B, C, D four treatment groups with six replicates of 10 broilers. The control group that group A was fed the basal diet without sodium gluconate addition. For treatment group, sodium gluconate was added in feed at 0.1%, 0.2%, 0.3% of basal diet. The experiment aims to study the effects of sodium gluconate on growth and fatty acid synthesis of broilers.Results:1.Gastrointestinal Tract:1)Intestinal Structure There are significant differences between Group B(0.1% sodium gluconate) and control group on V/C at 21 days(P<0.05). The other treatment groups have no significant difference with control group on V/C(P>0.05).The V/C of each treatment group were higher than control group and showed a decreasing trend. Gut Microflara There are significant differences between Group B(0.1% sodium gluconate) and control group on V/C at 42 days(P<0.05). The other treatment groups have no significant difference with control group on V/C(P>0.05).The V/C of each treatment group were higher than control group and showed a decreasing trend.2)Gut Microflara There were significant differences between treatment groups and control group on countss of lactobacillus at 21 days(P<0.05). And group B has the largest counts of lactobacillus There were no differences between treatment groups and control group on countss of lactobacillus at 42days(P>0.05). But the countss of lactobacillus of treatment groups were more than control group and showed a increasing trend.There were significant differences between treatment groups and control group on countss of bifidobacterium at 21 days(P<0.05). And group B has the largest counts of bifidobacterium There were no differences between treatment groups and control group on countss of bifidobacterium at 42days(P>0.05). But the countss of bifidobacterium of treatment groups were more than control group and showed a increasing trend.There were no differences between treatment groups and control group on countss of E.col at 21days(P>0.05). But the countss of E.col of treatment groups were lower than control group and showed a decreasing trend. There were significant differences between treatment groups and control group on countss of E.col at 42days(P<0.05). And the countss of E.col of treatment groups were lower than control group and showed a decreasing trend.2.Microbial Cultures1)Effects of Different Concentrations of Sodium Gluconate for E. ColCompared with the control group, the counts of E. coli of each sodium gluconate group were significantly different(P <0.01). And the countss of E. coli of contol group higher than sodium gluconate groups. The group with 0.1% sodiumgluconate has lowest countss of E.Col.2)Effects of Sodium Gluconate as Sole Carbon Source for E.ColThe counts of E. Col in control group higher than sodium gluconate(P >0.05).3)Effects of Sodium Gluconate as Sole Carbon Source for LacLac can not survive in the sodium gluconate medium.3.Growth Performance Compared with control group,the weight and weight gain of group B has significant increase(P <0.05) at 42 days. At 22-42 days, feed intake and feed conversion ratio of treatment groups lower than the control group and have decrease trend(P>0.05).4.Atty Acid Synthesis The abdominal fat rate of each treatment group were lower than control group by and showed a decreasing trend( P>0.05). Group C decreased 11.71% than control group.5.Immunion Performance The speen index and IL-2 m RNA expression of Group B(0.1% sodium gluconate) was significant higher than control group(P<0.05).