Fragrance Component Analysis And Gene Cloning In Narcissus Tazetta

Narcissus tazetta L. one polyanthous and fragrant aroma plants,it belongs to the family Amaryllidaceae. The fragrance is very important for ornamental plants, the fragrance of Narcissus is complex, it included esters, terpenes, aldehydes, alcohols, and other compounds. In this study, we component analysis the fragrance and clone the gene in Narcissus,So it provides a reference for the fragrance compounds exploit and a certain theoretical basis and technical support for ornamental research on genetic engineering of Narcissus.The fragrance compounds at different stage and part of flower was identified by gas chromatography linked mass spectrometry (GC-MS) in some Narcissus tazetta.The trans-ocimene and benzyl acetate were the most compounds in Narcissus tazetta,so them may play a important role for fragrance.Two HMGS genes were cloned from Narcissus tazetta var. HUANGHUASHUIXIAN II and JINZHANYINTAI by SSH and RACE. The ORF encompassed 1413bp encoding a polypeptide of 470 amino acids, and there are two amino acids different between them. Sequencing analysis showed that the amino acid sequence of Narcissus tazetta was 83%、82%、82%、81% homologous with HMGSgenes from Vitis vinifera, Glycine max, Ricinus communis, Zea may respectively. The relative expression levels of HMGS at two varieties were analyzed by qRT-PCR and NtActin was used as reference gene. The qRT-PCR results showed that HMGS relative expression levels at the two varieties are not obvious differential.Two WD40 genes were cloned from Narcissus tazetta var. HUANGHUASHUIXIAN II and JINZHANYINTAI by SSH and RACE. The ORF encompassed 1302bp encoding a polypeptide of 433 amino acids, and there are three amino acids different between them. Sequencing analysis by BlastX showed that the amino acid sequence of Narcissus tazetta was 74%、73%、73%、73% homologous with WD40genQS from Arabidopsis lyrata, Glycine max, Zea may, Ricinus communis respectively. The relative expression levels of WD40 at two varieties were analyzed by qRT-PCR and NtActin was used as reference gene. The qRT-PCR results showed that WD40 relative expression levels at the two varieties are not obvious differential.