OsCBSCBSPBI Regulate Drought-Tolerant Characteristics Of Rice In Reproductive Period And Functional Study

Drought is a serious problem to threat to rice, they will bring largely influernce of the growth stage of rice, the effect of reproductive rice directly led to the huge production precipitously under the drought stress. So the research of related drought gene in reproductive period is extremely urgent to improve ability of drought stress of rice. Reserch shows that gene blongs to the superfamily comprised two cystathionine beta synthase domain and one PBI domain. The family may be widely participate in the plant abiotic stress response, so the function of the gene related to parsing mechanism of rice drought resistance is of great significance. This experiment with wild type and T-DNA insertion mutants as material, and analysis when drought stress the reproductive function of oscbscbspbi and how to regulate and control. The result shows that:(1) Bioinformatics analysis that OsCBSCBSPBI gene in rice genome located in chromosome 1,contains 14 exons, and encoding 238 amino acids, through bioinformatics software analysis found that the size of protein is 23.7 kDa, isoelectric point is 9.89, this gene contains two CBS domains and one PBI, homology comparison of Amino acid found that the gene is very conservative. evolutionary tree shows that compared with rice genetic relationship of species is millet and sesame seeds respectively.(2) 5 days later with Drought treatment found that zhonghua 11 be considerably higher than oscbscbspbi in root activity of rice. The relative water content of root、stem、leaf and anther of zhonghua 11 are significantly higher than oscbscbspbi.At the same time we observed that the pollen sterility and activity in rice in drought treatment before and after, whether both pollen sterility and activity, zhonghuall are rather higher than oscbscbspbi, after the transgene into the oscbscbspbi, the expression quantity of oscbscbspbi are the same as zhonghua 11, even surpassed zhonghua 11. drought torlerent ability of oscbscbspbi are raised similar to zhonghua 11, it confirmed that the gene is likely related to drought torlerent in rice. The above results showed that the gene directly influenced the drought resistance ability of the rice in generative phase.(3) Studies have shown that the gene directly influence the expression of protective enzymes and membrane damage. Protection enzymes, such as superoxide dismutase (SOD)、glutathione (GSH)、osmotic protection substances such as proline (Pro)、soluble protein and soluble sugar are rising, and zhonghua 11 rised higher than oscbscbspbi significantly, membrane damage substance such as malonaldehyde (MDA) and TBARS, by contrast, oscbscbspbi were significantly higher than zhonghua 11.(4) Tissue specificity study showed that the gene expression mainly concentrated in the anther. The second is also expressed in the leafs. OsCBSCBSPBI gene subcellular localization results show that the OsCBSCBSPBI gene coding protein is a kind of membrane proteins, is likely to be involved in plant adversity stress signal transduction and protective cell members from damage.(5) The expression analysis of gene:after drought treatment, zhonghua 11 and oscbscbspbi’s ABA in the dynamic changes of the expression is differ, The result shows that the genes may be regulating the drought-resistant ability by relying on ABA signaling pathways. After the genetically modified, The expression of oscbscbspbi are closed to the zhonghua 11, and exceed zhonghua 11 slghtly. Confirmed that the gene probably correlated with drought resistance in rice, DERB1A and DERB1E belongs to DREB1/CBF family, AP59 and AP37 to AP2/ ERF family, a rise in the gene helps increase the drought-resistant ability of plants. After drought treatment, the content of these genes of zhnghua 11 are all higher than oscbscbspbi, drought-resistant ability of zhonghua 11 are significantly stronger than oscbscbspbi. these Genes associated with ROS way.such as APX2、SodA1、Cat can degrade the ROS, thus it can inhibit the accumulation of ROS, the study also found that the expression was significantly higher in zhonghua 11, it showed that the gene likely to be involved in cell pathways to enhance the ability of drought-resistance of ROS. By looking for KEGG, OsCBSCBSPBI gene may arise through degradation of homocysteine cysteine and glutathione synthesis to remove active oxygen and free radicals in cells. Detailed cellular signaling pathways and mechanisms remain to be research.