The Regeneration Of Microspore Embryoid And The Difference Analysis Of DH Lines In Cabbage(Brassica Oleracea L.)

The research aims to improve the efficiency of direct regeneration of cabbage embryoid and to enhance the growth vigor of DH plants of cabbage, for getting DH lines of cabbage more, better, faster, and accelerating the breeding process of cabbage DH lines. In this study, the medium type, the mass concentration of agar, the low temperature treatment, the concentration of melatonin, the mass concentration of brassinolide have been researched to improve the efficiency of direct regeneration of cabbage embryoid, and the concentration of NAA with different medium, the illumination, the mass concentration of NH4NO3 to enhance the growth vigor of DH plant of cabbage. Moreover the difference analysis of cabbage DH lines was also studied. The results are as follows:1. All of the experiment, low temperature treatment and the medium added different mass concentration of agar and different concentration of melatonin, screened the result that the medium of B5 is better than medium of MS. The suitable medium for direct regeneration of cabbage embryoid is the B5 medium.2. The embryoid transferred to the medium added agar12 g/L, the result of cabbage embryoid turned green and grow out leaves performed best, the number of direct regeneration of embryoid and the number of plant regeneration is the most and the growth vigor of plants performed best.3. The embryoid in the treatment of 10℃ and 4℃ of 3 d and 6 d, the efficiency of direct regeneration of cabbage embryoid performed better. The number of plant regeneration performed very significant difference while embryoid in the treatment of 4℃ in 6 d.4. The number of direct regeneration of cabbage embryoid and the number of plant regeneration is the most. The embryiod grew better and the time of embryoid grow out leaves is shorter in the treatment of medium added melatonin.5. The medium added brassinolide 0.075,0.15,0.3 mg/L, finally, almost all of the embryoid transformed into callus and few callus transformed into buds.6. The DH plants, which were grown in 1/2MS medium added 0.1 mg/L NAA, had the fastest growth rate and strong growth vigor,rooting fast7. Cultured in the illumination of 80 μmol/(m2·s),the DH plants had the most remarkable growth speed, better roots and leaves, which rooted in 10.3 days8. The DH plants in MS medium with added 0.4 g/L NH4NO3 had the maximum value 56.25 mm of net growth height in 35 days.9. Compared in two cabbage DH lines, the DH1-2 had the smaller difference than DH1-1. The DH lines had the smaller difference while compared with multi-generation inbred line. DH1-2 had the minimum of 5.56% in head color and the DH1-1 had the second minimum of 10.00% in head color. The S1 line had the difference in color of exterior leaves and head color respectively is 3.85% and 15.39%.