The Application Research Of Non-starch Polysaccharide Enzymes In Barley-DDGS Diet Of Broiler

Nine hundred and sixty 1-day-old AA broilers were randomly divided into 8 treatments with 6 replications(20?6?8=brids?replications?treatments). The group 1 fed a regular energy diet as positive control group; the group 5 fed an energy-restricted diet that 60 Kcal energy less than the conventional diet as a negative contrast group, the group of 2-4 added 100, 200, 300 g/t of non-starch polysaccharide enzymes on the basis of positive control group, and the group of 6-8 added that on the basis of negative contrast group. Throughout a breeding cycle for 42 days, the animal experiment was designed to assess the effect of non-starch polysaccharide(NSP) enzymes on viscosity and pH of Intestinal Chyme, performance, digestive organ and nutrient utilization of broiler in the barley-DDGS diet, which provided scientific basis for the researchers and feed enterprise choosing NSP complex enzymes.Experiment 1 Effect of non-starch polysaccharide enzymes on performance and nutrient digestibility of broiler in the barley-DDGS diet. The results showed as follows: 1)In 1~21d, ADFI of the group 8 was significantly lower than the negative contrast group 5(P<0.05);In 22~42d, the ADFI and F/G of the group 4 were significantly lower than that of the positive control group 1(P<0.05); In 1~42d, the ADFI of group 4 was significantly lower than that of group 1(P<0.05) and that of group 8 was significantly lower than group 5(P<0.05). The optimal supplementation of NSP enzymes in a barley-DDGS diet for broilers was 300 g/t on the performance improvement 2)in 1~21d,the protein utilization ratio of group 8 was significantly higher than group 5(P<0.05);in 22~42d, the protein and energy utilization ratio of group 8 were significantly higher than that of groups 5(P<0.05).The optimal supplementation of NSP enzymes in a barley-DDGS diet for broilers was 300 g/t,which the nutrient nutrient utilization raised most obviously.Experiment 2 Effect of non-starch polysaccharide enzymes on viscosity and pH of Intestinal Chyme and digestive organ of broiler in the barley-DDGS diet.The results showed as follows: 1) in 1~21d, the relative weight of glandular stomach of group 4 was lower than that of group 1(P<0.05); in 22~42d, the enzymatic groups have a tendency to reduce the relative length of duodenal(P=0.062). The relative weight of jejunum and ileum in group 4 were significantly lower than that in group 1(P<0.05) and that of group 8 was significantly lower than that of group 5(P<0.05).The optimal supplementation of NSP enzymes in a barley-DDGS diet for broilers was 300 g/t,which made the favorable effect of digestive organs best. 2)in 1~21 d,the viscosity of small intestine in group 7 and group 8 were significantly lower than that in group 5(P<0.05); the enzymatic groups have a tendency to reduce the pH of Duodenal and ileum(0.05<P<0.10).in 22~42 d, the viscosity of small intestine in group 8 were significantly lower than that in group 5(P<0.05); the pH of ileum in group 4 was significantly lower than that in group 1(P<0.05) and that of group 8 was significantly lower than that of group 5(P<0.05). The optimal supplementation of NSP enzymes in a barley-DDGS diet for broilers was 300 g/t, which the viscosity and pH of Intestinal Chyme decreased most obviously.According to the results of this study, the NSP enzymes in conventional and low energy(low 60 kcal) barley-DDGS diet improved the ADFI, ADG and F/G of broiler by different degrees, significantly improved the nutrient utilization and digestive organs, and the viscosity and pH of Intestinal Chyme of broiler decreased significantly.The optimal supplementation of NSP enzymes was 300 g/t in the condition of this experiment.