Proteomics Analysis Of Cotton And Whitefly Interaction And Functional Analysis Of GhMLP423 Gene

As one of the most important economic crops in China, cotton always has been confronted with a serious threats from all kinds of pests during the long producting process. In recent years, whiteflies occur frequently in many cotton-growing areas, which has brought seriously harm to cotton production and fiber quality. The mechanism of the response to whiteflies stress on the cotton is still unclear.We analyze the difference of all expressed proteins between the strong whitefly-resistance cotton variety(JH) and the susceptive cotton variety(ZS) by means of proteomics. We have identified 263 proteins successfully by mass spectrometry, and there are 176 specific proteins for analysis after removing redundant. We find that a considerable part of proteins are involved in the oxidation-reduction reaction and some are the enzymes associated with the active oxygen signal path by enrichment analysis and metabolic pathway analysis. After detecting enzyme activity of resistant material JH, the enzyme activity of SOD is significantly higher than the control so that the hydrogen peroxide content is increasing the initial and then becoming to decrease gradually; while peroxidase activity reduces significantly. These results suggest that reactive oxygen signal path may be involved in the process against the whitefly infestation in cotton. The protein expression level of GhMLP423 gene increased in cotton being infested by whitefly. The peroxidase activity in the cotton of interfering GhMLP423 is lower than the control, and the enzyme activity of SOD is higher than the control so that the superoxide ions is induced into hydrogen peroxide; hydrogen peroxide content is initially higher than the control and then become to decrease gradually. SA content is less than normal plants in leaves of GhMLP423 interference lines. After whitefly infestation, SA content in normal plants is significantly higher than it in non-infected plants, while the hydrogen peroxide content is also lower than it in normal plants, both SA content and the hydrogen peroxide content increase in both non-infected plants and normal plants being infested by the whitefly. The hydrogen peroxide content in GhMLP423 interference lines is also significantly lower than it in the control. It may indicate the accumulation of SA inhibits catalase activity when the cotton are infested by the whitefly so that it begin to accumulate hydrogen peroxide. Compared with the control plants, there is no accumulation of ABA and JA in GhMLP423 interference lines and the plant shows more susceptive for whitefly, indicating that GhMLP423 gene may be involved in the transduction process of JA signal path.We analyze the proteome of different resistant cotton variety being infested by the whitefly and screened resistance genes of cotton against the whitefly. Meanwhile we analyze the physiological index of cotton in order to reveal further the effect of related proteins in the cotton against the whitefly. We hope that provide some new ideas for the mechanism of cotton to insect-resistant.