Detection And Verification Of QTL On Silique-traits In Brassica Rapa Using RILs And CSSLs

Silique length (SPL), multilocular silique (MS) and seed number per silque (SSN) are three important yield-related traits controlled by quantitative trait loci (QTL) in oil seed rape (Brassica napus L.). In this study, we conducted QTL analyses for SPL, MS and SSN using 124 recombinant inbred lines (RILs) and 132 chromosome segment substitution lines(CSSL). RIL and CSSL were grown in Shengyang agricultural university in the glass greenhouse and plastic greenhouse. Main results were given below.1.The software QTL IciMapping was used to conduct the composite interval mapping. We conducted QTL analyses for SPL, MS and SSN using 124 recombinant inbred lines (RILs) and 132 chromosome segment substitution lines(CSSL). A total of 57 quantative trait locis (QTLs) were detected in the RIL population and CSSL population. They were not evenly distributed. There was no detection on A8 linkage group. These consensus QTL individually explained 0.12%-97.42% of phenotypic variation.2.A total of 22 quantative trait locis (QTLs) were detected in the RIL population. For the focusing trait in this study, the number of QTLs identified about silique length, was 2 and 1 in glass greenhouse and plastic greenhouse, respectively. One QTL was repeatedly detected in two environments. Seed number per silque, the number of QTLs identified was 6 and 5 in the two environments, respectively. Four QTL was repeatedly detected in two environments. Multilocular silique, the number of QTLs identified was 3 and 5 in two environments, respectively. One QTL was repeatedly detected in two environments.3.A total of 35 quantative trait locis (QTLs) were detected in the CSSL population. For the focusing trait in this study, the number of QTLs identified about silique length, was 2 and 2 in both environment, respectively. One QTL was repeatedly detected in two environments. The number of QTLs identified about seed number per silque, was 1 and 2 in two environments, respectively. One QTL was repeatedly detected in two environments. The number of QTLs identified about multilocular silique, was 18 and 10 in two environments, respectively. Seven QTLs was repeatedly detected in two environments.4.On the A2 linkage group, we detect a main effect QTL controlling the silique length, 28.81%of the site of the contribution rate, and BrID90021 close chain. On the A9 linkage group we detect a main effect QTL controlling the multilocular silique,97.62%of the site of the contribution rate, and BrID 10435 close chain.