The Protective Effects Of Genistein On Bisphenol A Induced Liver Injury In Mice

Objective:Both of environmental endocrine disruptors BPA and phytoestrogens genistein can combine with the estrogen receptor, but the biological effects they produce are not quite the same. In this study, to examine the damage effects of low concentration of BPA on the liver and to investigate the protective effect of genistein on bisphenol A induced liver injury, we use mice as the experimental animals and establish the BPA and genistein exposure model, to provide the scientific basis of the mechanism of BPA induced liver injury and the protective effect of genistein on this liver injury in mice.Methods:Randomly selected 96 4-week-old healthy male Kunming mice and divided into 16 groups of six. Corn oil was used as the solvent and made four doses of BPA and four doses of genistein, made groups by combining different does of BPA and genistein. Experimental animal models were established the by daily gavage for 56 days. After the exposure, mice were weighed and blood was collected, then serums were isolated for biochemical indicators detection. Liver tissues were collected and weighted to calculate the liver index. Histopathological changes were observed after HE staining, the remaining tissues were prepared the liver homogenates. Detected the vitality of alanine aminotransferase(ALT/GPT) activity and aspartate transaminase(AST/GOT), and the concentration of albumin(ALB) and total protein (TP) in the serum, and the activity of superoxide dismutase(SOD) and malondialdehyde(MDA) content in liver homogenates, then analyzed.Results:1. BPA exposure caused liver index increased, and genistein reduced the liver index BPA exposure in mice.2. BPA exposure caused morphology changes in mice liver, with the dose of BPA increased, the arrangements of hepatocytes were more disordered, cell boundaries were not clear, cell necrosis were increased; genistein could reduce the injury of BPA on the liver.3. BPA exposure increased the ALT and AST vitality and decreased ALB and TP content in mouse serum, and the differences were statistically significant compared with the control group. Compared with the same does of BPA group, serum ALT and AST vitality were decreased with the increasing doses of genistein, and the serum ALB and TP content were increased with genistein and the difference was significant with high doses of genistein.4. BPA increased the activity of SOD and decreased the content of MDA, but compared with the same does of BPA group, genistein decreased the activity of SOD and increased the content of MDA after BPA exposure.Conclusions:1. There is a positive correlated with the dose of BPA and morphology changes in mice liver, and genistein has a protective effect on liver morphology changes which induced by BPA in mice.2. The liver damage induced by BPA can increase the vitalities of ALT and AST in mice serum, but decrease the ALB and TP levels, resulting a dysfunction in liver, and genistein has protective effect on this.3. The liver damage induced by BPA may be due to the tissue oxidative stress with a decreased SOD activity and an increased MDA content in liver after BPA exposure, but genistein can increase the activity of SOD and decrease the MDA content in liver tissue, and give protective effect of liver from the aspect of oxidative stress.