The Expression Of FGFRs In Different Tissues Of Laying Hens

Fibroblast growth factor receptor (FGFRs) is a kind of transmembrane protein with a high degree of homology in structure, and belongs to the family of receptor tyrosine kinases. FGFRs include four types, namely FGFR1-4, which participate in the regulation of various physiological functions of the body, including embryonic development, skeletal development and formation, calcium and phosphate homeostasis, cell proliferation and differentiation, and the occurrence of tumor. But the current researches of FGFRs focused on human and mouse, there have few detailed informations on the distribution and role of FGFRs in poultry, especially in layers. In this study, the expression of FGFR1, FGFR2, FGFR3 and FGFR3 mRNA in laying hens kidney, muscle (pectoral muscle and skeletal muscle), bone, small intestine, adrenal gland, parathyroid gland, fallopian tube, ovary, hypothalamus, pituitary and heart tissues were detected by RT-PCR method, and the expression of FGFR1 and FGFR2 protein in the above tissues also were detected by Western-blot technique. The results will provide the basis for further investigating the role of FGFRs in laying hens.The results showed:FGFR1, FGFR2, FGFR3 and FGFR4 mRNA were expressed in kidney, muscle (pectoral muscle and skeletal muscle), bone, small intestine, adrenal gland, parathyroid gland, fallopian tube, ovary, hypothalamus, pituitary and heart of laying hens. Both FGFR1 and FGFR2 protein expressed in laying hens kidney, muscle (pectoral muscle and skeletal muscle), bone, small intestine, parathyroid, fallopian tube, ovary, hypothalamus, pituitary and heart; however, there were no expression in the adrenal gland.The results indicated that:fibroblast growth factor receptor (FGFRs)-FGFR1、FGFR2、 FGFR3 and FGFR4, especially FGFR1 and FGFR2, express in laying hens kidney, muscle, bone, small intestine, adrenal gland, parathyroid gland, fallopian tube, ovary, hypothalamus, pituitary and heart tissues, and it may be involved in the regulation of these organizational functions, but its exact role needs to be further investigated.