Taeniidae Family Infection In Wide Life Definitive Host Species, Tibetan Fox (Vulpes Ferrilata)

Taeniidae, consisted of two valid genera Taenia and Echinococcus, causes lethal zoonotic taeniasis/cysticercosis globally. These diseases mostly prevalenting in rural populations of pastoral and mountainous in northern hemisphere bring serious threaten to local human beings and domestic animals. In China, taeniasis /cysticercosis is prevalent in most districts, especially in Qinghai-Tibetan plateau. But, researches were concentrated in the infection of human being and livestock in the synanthropic partten transmission.Tibetan fox (Vulpes ferrilata) was known as an important definitive host of Echinococcus. However, the infection of taenia species, even the whole Taeniidae were unknown in Tibetan fox. Research of Taeniidae in Tibetan fox can help us understand the transmission mechanism of Taeniidae in field environment. On that basis, effective measures can be taken to prevent the taeniasis/cysticercosis.DNA barcoding has been proved useful in identifying and classifying numerous species at the same time. But its effectiveness in fecal samples is unknown.In our study,37 fecal sample which collected in Sichuan province Shiqu conty in Qinghai-Tibetan plateau were used to detect the Taeniidea parasites. Cropo-DNA were extracted and identified their host species. And then, DNA barcoding and species specific primers PCR were taken to detecte the Taeniidae species in 37 fecal samples. Echinococcus multilocularis, Echinococcus shiguicus, Taenia hydatigena, Taenia polyacantha and Taenia asiatica are found. At the same time, a gradient test was revealed to modify the fecal environment to evaluate these primers of two methods. The result showed that there were no significant difference between these methods.