Studyies Of Sucrose Metabolism Changes In Chinese Olive Fruit Mature Development

The contents of sucrose, glucose, fructose, total sugar, activities of sucrose-metabolizing enzymes and mineral elements in the Chinese olive fruits of ’Makeng 22’and’Tantou 23’were compared. Results showed that:1. The search establishes isocratic elution method of HPLC to get the contents of sucrose, glucose and fructose. The results of parallel determination sugars from sample show that relative standard deviation is 0.50%-4.43% and recovery is 96.76%-106.29%. Therefore this test method own good reproducibility, and results are reliable. Then isocratic elution method can be used in Chinese olive fruit sugars content determination.2. Sucrose accumulation of Chinese olive fruit can be rough divided into two stages:in the early stage sucrose accumulation is slow, difference of which between ’Makeng22’and’Tantou23’is not significant. The late stage is sucrose rapid accumulation period, which was the key to sucrose accumulation difference between ’Makeng22’and’Tantou23’,and it also affected soluble sugar content of fruit when Chinese olive fruit was ripe. The ratio of hexose to sucrose was 0.668 in the ripe ’Makeng22’fruit. The ratio of hexose to sucrose was 0.904 in the ripe’Tantou23’ fruit.3. In sucrose rapid accumulation period, AI activity of Makeng22’was higher than’Tantou23’, which created a chance for sucrose rapid accumulation. And NI activity of’Makeng22’was lower than Tantou23’, which strengthened the fruit sink. SPS activity changes of’Makeng22’ and ’Tantou23’were similar, so SPS was not the key to the sucrose accumulation. The growth of ’Makeng22’fruit SuSy (synthesis direction) activity was higher than Tantou23’ in after full bloom 144 d-186 d, so SuSy (synthesis direction) was the key to the sucrose accumulation. Besides, sucrose rapidly accumulating relied mainly on SuSy synthesis direction activity changes to promote the increase of sucrose content in ’Makeng22’fruit. Sucrose rapidly accumulating relied mainly on SuSy cleavag-e direction activity changes to promote the speed of sucrose into fruit in’Tantou23’fruit.4. In addition to calcium, mineral elements of different strains of Chinese olive fruit sugar accumulation process quality effect is roughly same, including the quality effect of the potassium the most significant. Potassium within the fruit on the activation of SPS activity has certain effect, which can promote the rapid accumulation of sugar content in fruit ripening process. Phosphorus is conducive to the accumulation of sucrose content, especially sink of ’Makeng22’ significantly stronger. Magnesium can inhibit the accumulation of glucose and fructose, which may contribute to the consumption of sugar in Chinese olive mature development. There is not significant correlation between nitrogen, zinc and sugar metabolic. The correlation of Ca element and sugar metabolic is obvious difference between ’Makeng 22’ fruit and’Tantou 23’fruit, the main reason of which may be that different forms of Ca element play different roles in Chinese olive mature development.