Metabolic Impact On Magnaporthe Oryzae And Its Host After Deletion Of MoATG Genes And Screening Of Different Metabolites

The deletion of ATG genes impacts on autophagy of fungi, meanwhile increases or decreases the content of metabolites in fungi. A host-pathogen interaction turns out to be highly specific, and a large amount of metabolites can be produced in this process. Accordingly, we studied on the interaction by detecting the changes of these metabolites. Impairment of autophagy in Magnaporthe oryzae, by the targeted mutation of the MoATGl gene, rendered the fungus nonpathogenic. Deletion of MoATG11 gene in M.oryzae has no influence on autophagy. Furthermore, △Moatg11 mutant and the wild-type guy11 strain were both able to cause severe disease symptoms. △Moatg15 was highly reduced in virulence to rice. To learn more about the genetic functions of ATG genes in M.oryzae, we used metabolomics technology, which combined GC-MS、LC-MS with mutivariate statistical methods such as principal component analysis(PCA) and orthogonal partial least squares discriminant analysis(OPLS-DA), to research on wild-type guyll strain and AMoatgl, △Moatg11, △Moatgl5 mutant strains of M.oryzae along with rices inoculated with these strains. The main contents as follows:(1) There were 43 different metabolites, related to deletion of MoATGl、MoATG11、 MoATG15 in M.oryzae, identified. The metabolic network affected by MoATGl, MoATG11、 MoATG15 was constructed and many metabolic pathways involoved, including citrate cycle, glycolysis, vitamins metabolism, amino acid metabolism and secondary metabolism. We finally elaborated these pathways and the biological significance of them.(2) There were 45 different metabolites, related to the response of rice to infection of M.oryzae, identified. Combining with the pathogenic phenotypes of rice leaves, we found that the different responses of host plants can be attributed to different virulence of pathogens with various genotypes. We used weight analysis to analyze the metabolic pathways of rice related to the 45 metabolites and found the most relevant pathways were alanine, aspartate and glutamate metabolism, glycine, serine and threonine metabolism, glyoxylate and dicarboxylate metabolism, inositol phosphate metabolism and phenylalanine metabolism. Then the metabolic network was constructed in a framework that erected by the 5 main pathways and biological significance of them was also illuminated.This study will provide some theoretical evidence for investigating the genetic function of MoATG genes in M.oryzae systematically and comprehensively, target screening and drug verification related to autophagy of M.oryzae, developing new fungicides aimed at decreasing the occurrence and harm of M.oryzae to host plants and thereby increasing yield and quality of plants.