Effects Of Pig Manure On Antibiotics Behaviors In The Farmland Ecology Environment

Pollution of farmland ecology environment by antibiotics is showing up as wide application of the antibiotics in breeding industry. Antibiotics pollution of farmland cannot only influence the soil microbial diversity, but also increase the resistance of soil microbial to antibiotics by impacting on soil enzyme system and NPK circulation. However, previous studies have focused on the effect of types and concentration of antibiotics on farmland ecology environment. There are fewer researchers studying antibiotics’s effect when animal manure is together applied. Therefore, laboratory simulation experiment and field test were carried out to study the adsorption and degradation of antibiotics in the soil with or without application of pig manure, and discuss the antibiotic effects on soil microorganism and nutrient transformation, and explain the impact of uptake and accumulation of antibiotics in vegetables after pig manure was applicated. At the same time, the preliminary experiment also analysis the effect of combined antibiotics pollution on soil microbial. Main results obtained in the study were presented as follows:1. Adsorption capacity of 4 representations of antibiotics in soils: oxytetracycline> enrofloxacin> tylosin> sulfamethazine. Application of pig manure can increase the intensity of soil adsorption of antibiotics, and decrease antibiotic activity in the soil, and thus weakens effects of antibiotics on the ecological environment.2. Application of pig manure can accelerate the antibiotics degradation in the soil. The degradation rate increased with the increase of consumption of pig manure, especially the early stage of manure application. Terramycin, Tylosin, sulfamethazine and enrofloxacin degradation rates increased by 7.90%～8.80%,6.42%～7.21% 7.91%～9.33% and 8.36%～12.54% under the 1.0% pig manure application as compared with the control (no application of pig manure).3. In the case of no pig manure application, high concentrations of antibiotics can decrease the microbial biomass carbon and microbial quantity of soil and soil available nitrogen (NH4+-N, NO3-N) and urease activity of invertase and catalase. However, the cases of application of pig manure, the inhibition on the soil microorganism significantly weakened under the high concentrations of the antibiotic, while effects on soil enzymes changed slightly. Microbial quantity, microbial biomass carbon, enzyme activity and nitrogen availability (NH4+-N, and NO3--N) is controlled by the consumption of pig manure.4. Application of pig manure can increase soil microbial biomass carbon, promote degradation of soil antibiotics, reduce the soil antibiotics absorption of vegetable, the degradation increased when the consumption of pig manure increased. Meanwhile, contents of antibiotics of roots were higher than the aerial parts significantly.5. Under indoor simulated conditions, single or combined pollution of antibiotics all had an impact on soil microbial diversity. As opposed to single pollution, effects of combined pollution on soil respiration are not obvious, but effects on soil urease activity enhanced and effect on the activity of alkaline phosphatase and catalase is not significant. High-throughput sequencing showed that the soil microbial community structure is changed by antibiotics significantly. According to different antibiotics, the effect of combined pollution of antibiotic on soil microbial diversity is not common. Its mechanism need to be further explored.