Clinical Application And Dose-Exploration Of Midazolam On Ca²⁺Pathway

Purpose: The purpose of this dissertation was to investigate the effects of injection of different doses of midazolam on Ca2+-Ca M-Ca MKII path as well as study the changes in ion channel and related transduction factor contents. Midazolam inhibition of brain nerve center and its effects on learning and memory have been deeply explored in this paper for further clinical research and novel guidance of the rational useage of midazolam.Method: 72 male wistar rats were introduced in this dissertation. 24 rats were firstly selected by the author and divided into four groups for the Morris water maze test. Different groups of rats were immediately forced to take water maze test when they became awake after dosing in accordance with their respective requirements.This worked at the same time as planned each day.Rats got five-consecutive-day training after they could customize motions. On the same time of the sixth day, rats took space exploration test on the first second that they recover consciousness.Remaining 48 rats were randomly divided into four groups of 12. Four groups were further divided into four subgroups. Rats had to be decapitated after a variety of time. The expression of Ca MK and Ca MKII were detected by the method of RT-PCR. Ca2+ ion was expressed by colorimetric detection.Results: 1. Water maze results showed that compared to the controlled group, three groups of rats which were injected with midazolam showed longer escape latency in the navigation test. At the same time experiments in space exploration, the third quadrant dwell time ratio decreases, the results of going across the experimental platform also showed a dramatically decreasing trend.Furthermore, it could be seen that the greater the dose, the longer the average escape latency of rats crossing platform times and the lower the residence time ratios of third quadrant are. Each group was statistically significantly different(P<0.05). 2. Ca M and Ca MKII m RNA results of rat hippocampus tissues showed that midazolam dose-dependently inhibited the expression of Ca M and Ca MKII m RNA and the greater the dose, the more obvious inhibition by RT-PCR method.Each group was statistically significantly different(P<0.05). 3. The colorimetric determination of Ca2+ concentrations increases with the administered dose. Each group was statistically significantly different(P<0.05).