Research On The Correlation Of The Photosynthesis And Chlorophyll Fluorescence Of Rice Leaf

SPAD and chlorophyll fluorescence analyzers are fast, convenient and non-destructive, which provides the possibility for the simple measurement of physiological indexes of leaves. Through non-destructive spectroscopic measurements, it is possible to comprehensively analyze crop growth under different environmental conditions. The fluorescence analysis technique will become the useful tool and provide a strong technical support for the future development of precision agriculture. The purpose of this study was to combine the kinetics of fluorescence techniques and crop physiological and biochemical parameters analysis technology, and comprehensively analyze the indicators in the rice leaf growth period. Through the chlorophyll fluorescence parameters of quantitative analysis and an understanding for "immanence" of photosynthesis system in-depth, it will provide a theoretical basis for promoting modern agriculture and precision agriculture.In this study, rice #44 planted in Nanjing in Jiangsu Province was studied through field experiments. Based on fluorescence kinetics analysis, we studied the physiological and biochemical indicators of nature rice canopy leaves in each growth period over the seasonal changes. Through the analysis of fertility meteorological factors in 2014 and 2015,we concluded that temperature and moisture difference over two growth periods is relatively small, and the lighting conditions are quite different. The whole time is quite synchronized, but roughly it was planted earlier in 2014 than 2015 for a week or so. Under natural conditions throughout the growing period of rice, SPAD value and chlorophyll content, net photosynthetic rate and nitrogen content have a good relationship, but there is a certain degree of correlation of their differences. Among them, the relevance of SPAD value and chlorophyll content and nitrogen content of rice leaf is better, and the correlation between SPAD value and net photosynthetic rate is relatively different. Therefore SPAD value can be indirectly used to indicate rice canopy leaf photosynthetic capacity and nitrogen content. Studying on photosynthesis parameters found that in different periods which characterizes the strength of photosynthetic capacity of each growth in a certain extent, but also reflects the blade differences of different periods. With leaf senescence, the photosynthetic ability also decreased slowly, and each stage has its own salient features. On chlorophyll fluorescence analysis technique, the fluorescence is more complex and variable. In different growth period, fluorescence parameters Fo, Fm, Fv/Fm, Fo’, Fm’, Fv’/ Fm’ showed a high degree of consistency. Description in the absence of environmental stress conditions, changes in the value of these parameters is relatively small. By comparing ΦPSII and ETR, we found that electron transfer rate is not the same in different growth stages of rice canopy leaf. The latter significantly changed smaller, closely related to photosynthesis, so the photosynthetic rate was also significantly decreased. Chlorophyll fluorescence parameters separated the energy absorbed into three parts of P, D, E, so you can clearly see the proportion of P, D, E from the picture of area in different growth stages. In the early and mid-term, there is a larger proportion for photochemical energy response and non-chemical reaction energy dissipated less proportion. At the same time in different growth stages with the fluorescence intensity changes were measured and found photochemical quenching coefficients qP, non-photochemical quenching coefficient qN, the actual rate of chemical ΦPSII declines as the light intensity increases, but the rate is inconsistent. Contrary to the apparent electron transport rate, ETR increases with the light intensity. The development trend of two years is relatively consistent, so it can be modeled to achieve large-scale monitoring of satellite remote sensing to provide a theoretical basis. Using satellite remote sensing technology is relatively low-cost with real-time monitoring features and should be better implemented in a timely monitoring of environmental stress factors and condition assessment of plant pests in the field of applied research.