Study On The Characteristics Of Peel Oil Cell And The Expression Of Oil Cell Associated Gene On Kumquat

Kumquat is the fruit can be eaten with skin among citrus fruits. The size, amount and inclusion of oil vacuole on pericarp can directly affect the edible quality. Therefore, for the sake of the kumquat oil vacuole, this experiment will take a research on the fruit quality as well as the differences of oil vacuole’s size and amount through taking common kumquat Rongan with more oil vacuole, kumquat Huapi in bud mutation with less oil vacuole and kumquat Cuimi in bud mutation with least oil vacuole as the research materials. These three varieties of fruit transcriptome shall be sequenced and then through research on the differential gene, the relative controlling gene affecting the formation of oil vacuole can be analyzed. Besides, taking advantage of the expression quantity of measured selecting gen in each stage, the various expressions for these gens in the development process of fruit can be analyzed. What’s more, the connection between theses gens and the development process of oil vacuole can be predicated. It aims at providing a theoretical foundation for the cultivation of kumquat in high quality with less essential oil in oil vacuole in the applied genetic engineering.1.The number of oil cells have shown from initial growth among three kumquats. Within the fruit enlaring, the density of oil cell decreases slightly. In accordance with the amount of oil vacuole in unit pericarp area, the kumquat Rongan, kumquat Huapi and kumquat Cuimi shall be arranged in descending order. As for the kumquat Rongan and kumquat Huapi, the fruit density in ambitus section is the least and in the top one is the most.2. The advantages of kumquat Cuimi cover that it has large and heavy fruit, less oil vacuole, high edible rate, fruit juice percentage, soluble solid content, saccharose and contents of total sugar as well as low acid content. As for the flavor, it has the characters of less biting taste, crispy pericarp with more juice and thickness, which is sweeter with less sour taste.3. In total,36093 genes can be obtained through taking transcriptome sequence towards these three kumquat varieties. Compare the obtained gen with the protein database and then take annotation and classification of their functions. Through the comparison,20973, amounting for 58.11% exist in NR database. Besides, through GO, the obtained gen with functional annotation and predication reached to 20973, occupying 58.11% in transcriptome library.4.36093 genes in the three transcriptome libraries, such as kumquat Rongan (the control group), kumquat Huapi and kumquat Cuimi have been take the comparative analysis on the expression quantity. Finally,8113 differential expression genes (DEGs) were obtained. Through comparison kumquat Rongan with kumquat Huapi,1417 DEGs took gene expression states increased and 277 in decreased. Besides, the comparison between kumquat Rongan with kumquat Cuimi is 3134 increased,1514 decreased. And the comparison between kumquat Huapi with kumquat Cuimi is 757 increased and 1014 decreased.5. Taking GO significant enrichment analysis among 8133 DEGs, the amount of GO term in three comparative groups of kumquat Rongan with kumquat Huapi, kumquat Rongan with kumquat Cuimi and kumquat Huapi with kumquat Cuimi are 12,28 and 22 relatively.6. Through the comparison with NR database,46 varieties transcription factor from 200 genes were decoded. As for the AP2/ERF and WRKY that participated in the regulation and control of the alkene materials, it had 4 AP2/ERF transcription factors in the comparison between kumquat Rongan and kumquat Huapi; 26 WRKY transcription factors in the comparison between kumquat Rongan and kumquat Cuimi and 18 WRKY transcription factors in the comparison between kumquat Huapi and kumquat Cuimi.7. Select 5 regulatory enzyme genes and 5 transcription factors among the obtained 135 DEGs that can be likely decode essential oil component of oil vacuole, such as iridoid and ketone. It can be predicted that the relevant control gene of kumquat oil vacuole may exist in the synthetic route of flavonoid organism. The Unigene0008513 can control the disintegration of naringenin-3 and dihydroquercetin and the Unigene0000766 can regulate and control the neomenthol dehydrogenase to disintegrate the neomenthol.