| In this study, two paired monokaryon strains of Volvariella volvacea were tested. PYd21 was denovo sequenced sequenced by Solexa genome analyzer technology and assembled by Soapdenovo. Then PYd15 was sequenced and assembled reference to the genome sequence of PY21 using the same methods.The results of sequencing showed that the total length of the assembled genome of V.volvacea was about 37Mb included 302 scaffolds and 2598 contigs and the GC content was 48.52%. According to the assembly result of V. volvacea genome denovo sequencing, the length of scaffol N50 and contigN50 was 499679bp and 469816bp respectively.With the software Augustus (version2.1) used specially for fungus, whole coding sequence was calculated that the size was 16.8Mb and there were 11,534 genes in these domain regions.2184 repeats were found making use of Tandem Repeat Find.1210 and 1352 transposition elements were predicated respectively by RepeatMasker and RepeatProteinMasker. At last,4 rRNA genes、176 tRNA genes and 46 snRNA genes were obtained by using BLAST and PRIDICTION.Compared with the reference sequence PYd21, re-sequencing analysis showed showed that PYdl5 contained there were 35389 SNPs and 1132 InDels mutation sites. These InDels distributed in 71 scaffolds while the scaffold30 had the most InDels(93).943 structure variations were detected by pari-end and the most (51) SV sites existed in scaffold 133.SV molecule markers were developed according to the sequencing data of PYd15 and PYd21, and then were used to construct a genetic linkage map, which included 10 linkage groups by using F2 population that consisting of 192 monokaryon trains obtained from PYd15 and PYd21. The total length of genetic linkage map was 411.61cM and average distance was 4.03cM between two adjacent markers. The length of linkage group ranged from 3cM to "118.2cM. Contrasted with solexa sequencing, we obtained a genome sequence (19.65Mb) which covered 52.8% of the whole genome and at last,we assembled 269 scaffolds of V. volvacea. |
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