Mapping Of Growth Habit Related Gene In Common Bean

Growth habit is an agronomically important trait associated with the domestication in common bean(Phaseolus vulgaris L.), which is susceptible to environment such as photoperiod. Growth habit has a great effect on plant height, flowering period, maturity, water-use efficiency and bean yield. It is a key trait for mechanized production and important breeding strategy. Therefore, to map the growth habit genes in common bean not only allow us for a better understanding of the domestication process, but also a manipulation of breeding efforts of common bean.The gene was preliminary mapped on chromosome 1, by BSA with SSR markers developed according to common bean genome. Then, we completed the gh-lze mapping by SSR and In/Del markers developed in the aimed region establishing the molecular foundation for clonging the gene. The results are as follows:1. Population construction and genetic analysisAn F2 population was crossed from LianziNo.1×F0404. The phenotype data of F1, F2, F2:3 was collected in Changping Beijing, Nanyang Henan, Harbin Heilongjiang. F1 population was all indeterminate viny, and genetic analysis of F2 population presented a ratio of 3:1 between indeterminate viny and determinate bush, which indicated determinate bush was controlled by a single recessive gene.2. Polymorphic markers Screening310 Polymorphic markers were screened from a total of 2,802 SSR developed according to genomic sequences. Another 19 polymorphic markers were screened from 55 SSR aiming the primary mapping location for further mapping. We sequenced 15 candidate genes, according to polymorphsim segment, a total of three In/Del markers In87, In89, In89 have been developed as to gh-lze mapping growth habit gene, they are sited on 45,513,666 bp、45,532,820bp、45,575,103 bp on chromosome 1, respectively.3. Primary mapping of target genesThrough 310 polymorphic markers linkage analysis between recessive mixed pool and recessive parent, the target gene was maped on chromosome 1; Using the whole SSR markers on chromosome1 to genotype F2 popution, combined with the results of the phenotype and genetic map constructed by IciMapping v4.0, we found marker p1s86, p1s91 linked to target gene with genetic distance of 15.32 cM. Subsequently, p1s86 and p1s91 were positioned on reference genome and displaying to 2.5Mb region between two markers on the physical map.4. Gh-lze mapping the genesThe primary lactation region was narrrowed into 45,453,003bp~ 45,588,349 bp, by 19 polymorphic SSR markers. Three In/Del markers In87,In89 and In93 was used to detecte the recombinant in F2, the result showed that In93 detected only one recombinant, while In87 and In89 could not detected recombinant, explaining that In87 and In89 coseparate with target gene. So far, the growth habit locus was identified linkage with SSR marker p1t52 and In93 corresponding to a 122,100 bp on the physical map with the region containing 12 candidate genes, named Gene1~Gene12. The 12 candidate genes were annotated by BLAST searching NCBI, and Gene12 was annotated as TFL1 in common bean.