Effects Of 2 Kinds Of Compounds On The Physiology And Biochemistry Of 2 Kinds Of Plant Pathogens

Carbrone is a compound belongs to Sesquiterpene lactones and is mainly exists in the Asteraceae plants. The study found that the compound has wide antimicrobial spectrum. Through the analysis of the QSAR, the researchers found that the α-methylene-γ-butyrolactone is the main active group of carabrone. According to earlier research progress, we took the Carabrone and α-methylene-γ-butyrolactone as the tested compounds, discussed the influence of physiological and biochemical indexes of G.sanbinetti and G.graminis. The main results are as follows: 1. Use growth rate method to test wide antimicrobial spectrum of Carabrone and α-methylene-γ-butyrolactone, results showed that the two kinds of compounds have inhibitory activity on the growth of 10 kinds of pathogenic bacteria, and the inhibitory effect of the same compounds on the different pathogenic bacteria was significant. They both showed better inhibitory activity for G. sanbinetti and G.graminis, as Carabrone, EC50 is 37.628mg/L and 31.717mg/L; as α-methylene-γ-butyrolactone, EC50 is 92.202mg/L and 51.634mg/L. 2. Effects of Carbone and α-methylene-γ-butyrolactone on the content of ROS in mitochondria of pathogenic bacteria tested. Results showed that tested two compounds with G. sanbinetti and G.graminis, can cause the pathogen of mitochondrial ROS content increased significantly and obvious concentration dependence. This effect is particularly evident in G. sanbinetti, under the EC70 dose of Carbone and α-methylene-γ-butyrolactone, ROS content were 22.36 IU/mL and 32.89 IU/mL, Were significantly higher than the blank control treatment of ROS content. 3. Tested the effects of Carabrone and α-methylene-γ-butyrolactone in the two pathogens mitochondrial antioxidant enzyme activity. It was found that the CAT activity and GPX activity were significantly higher than that in the blank treatment after the treatment of the two compounds; The effect of the two compounds on the SOD activity of the two pathogens showed a phenomenon with increasing dose(EC50-EC70) activation followed by inhibition. 4. Determination of the Carabrone and α-methylene-γ-butyrolactone of oxalic acid and glycerol content. The determination showed that the content of oxalic acid decreased significantly after the compound treatment, the results showed that the two compounds could interfere with the synthesis of oxalic acid; Glycerol content decreased equally significant, the high concentration treatment showed lower glycerol content andα-methylene-γ-butyrolactone in EC50 and EC70 have been unable to detect two pathogens glycerol content. These two compound showed strong inhibitory effect.In summary, Carabrone can destroy pathogenic fungi of the antioxidant defense mechanisms, caused the accumulation of ROS, could inhibit important antioxidase SOD activity, destroy the structure of mitochondria in cells, reduce the pathogenicity of the pathogen; inhibit the biosynthesis of pathogenic bacteria, destruction of cell stable system, so as to achieve the purpose of inhibiting the growth of pathogens.