Exploring To The Avian Reovirus Nonstructural Proterin P17 Regu Latng C-myc Gene Signaling Pathway

Avian reovirus(ARV) belong to the reovirus are reovirus in the genera, poultry is an important pathogen. ARV infected poultry can cause a variety of disease and can cause serious economic losses, common viral arthritis/tenosynovitis, malabsorption syndrome(MAS), such as immunosuppressive diseases, but its pathogenesis is still know very little.ARV nonstructural protein p17 can participate in the nucleus of some reaction process, such as gene transcription, DNA binding and cell growth regulation, cell autophagy, cell cycle regulation, etc. C- myc gene regulation gene families as part of the cancer gene of nucleoprotein gene, the biochemical and cell proliferation, differentiation and induce apoptosis play a regulatory role in many aspects. Laboratory for the first time found the nonstructural protein p17 has influence to the c- myc gene expression, and early laboratory research ARV nonstructural protein p17 for cell autophagy, cell cycle and cell apoptosis research has made some achievements. This experiment was to study the ARV the nonstructural protein p17 for c- myc gene, gene expression regulation, and to further explore the ARV nonstructural protein p17 is through what kind of signaling pathways regulate the expression of c- myc gene, and the structural protein p17 for c- myc gene regulation mechanism of signal, and by using the signal path each key point to this specific blocker or shRNA inhibit the expression of proto-oncogenes, for ARV virus in the regulation of host cells provide a new research direction, and further used in the clinical prevention and control of ARV.1. Through the cold light test c- myc gene reporter in 0 h, 6 h, 12 h, 18 h and 24 h of the blank control group, infection ARV and transfection ARV nonstructural protein p17 group c-the strength of the active myc gene expression. Laboratory for recombinant plasmid PCDNA3.1- p17 and c- myc reporter plasmid transfection in DF 1, Vero and Hela, at the same time will ARV virus to 5 MOI(1.0 x 108 PFU/mL) access DF 1 respectively, Vero, Hela,uninfected ARV virus cells as blank control group. The results showed that the cells of the ARV infection group has the inhibition effect on the activity expression of c- myc gene, the nonstructural protein p17 inhibition activity of c- myc gene expression is more obvious. Cellsafter vaccination ARV 6 h, 12 h, 18 h measured c- myc gene activity compared with the control group did not have inhibition, but appeared in 24 h. The nonstructural protein p17 plasmid transfection cells after 18 h, 6 h, 12 h, 24 h test c- myc gene activity expression continues to abate, shows that the structural protein p17 showed inhibitory effect on the activity of c- myc gene expression.2. The early stage of the laboratory research has shown that ARV not structural protein structure protein p17 on AKT and mTOR have inhibitory effect, and is mediated through the ARV autophagy rely on PI3K/AKT/mTOR pathway. This experiment using Western Blot detection of structural protein p17 for c- myc inhibition of gene expression, the results show that p17 for c- myc gene expression suppression by PI3K/AKT/mTOR/FOXO1 signaling pathways.