| Mastitis is an important disease mainly infects goats, which could result in production and quality decrease of goat milk and great economic losses. Milk with low quality may cause offspring sick and even death. Livestock suffering mastitis may dead of toxin and systemic infection without timely and accurate diagnosis. Antibiotics are widely used for treatment of mastitis, but they may cause resistance strains existing and antibiotic residues in milk. So it is a good choice to use vaccine to prevent mastitis more than to use antibiotics for treatment.In this study, goats from Fuping, Qianyang, Dingbian, Hengshan, Jiaxian, Shenmu and Yuyang counties were investigated by using the method of california mastitis test(CMT) and inquiry. Staphylococcus aureus and Escherichia coli isolated from disease goats were cultured and inactivated by methanol with a concentration of 0.3% and then thoroughly mixed with aluminium hydroxide adjuvant with a proportion of 6:1(V/V). Goats were immunized with the inactivated vaccine and the titers of antibodies were assayed by the method of ELISA. The results were as follows:1. Respectively detected the recessive mastitis of 434 goats in Guanzhong district and165 goats from Yulin city of Shaanxi province and recorded the infection rate of the different district, the results show that the infection rate of the goat recessive mastitis in the Yulin region was 11.5%, the detection rate of the goat recessive mastitis in guanzhong region is34.1%;2. The antibody titer of goat mastitis inactivated vaccine were detected by ELISA after immunizing goats, results showed that antibody titers against Escherichia coli increased 9, 25 times separately in day14 and 28 with day 0; while Staphylococcus aureus were 21, 37 times in day 14 and 28. On the whole, the immunization level in goat was significantly better than the control(P <0.05). |
