Tissue Culture And Rapid Propagation Of Kiwi

Present kiwifruit varieties have been found up to hundreds of species, although kiwifruit variety of species, and each species has its own kiwifruit varieties features, but all aspects of good and suitable for cultivation, widely kiwifruit varieties in small quantities, because the kiwi dioeciously, by gender kiwi breeding offspring existence of gender separation, offspring instability was not fully retained excellent traits of the mother plant, therefore, to study cloning to breed excellent kiwi plants and kiwi tissue culture is the shortest period in each of asexual reproduction, the most efficient way. Tissue culture of high-quality resources to save, to lay the foundation for future cultivation of new varieties of kiwifruit excellent.The experiment selected the Actinida polygama, ‘Qihong’ kiwi,‘Qinmei’ kiwi umbilical three varieties of kiwi fruit with stem tip or bud stem section for the explant tissue culture system, establishment of test, studied the best way to disinfect 3 varieties of kiwi fruit, best time, original culture different hormone concentration and the ratio of the tube seedlings growth, the influence of the belt to cultivate kiwi different hormone concentration and three varieties were investigated and the influence of the ratio of tube seedlings growth, rooting cultivation of different hormone concentration and the ratio of the tube begins to take root, the influence of the tube seedlings transplanting when choose different cultivation matrix and the matrix of different ratio of the impact on the survival rate of seedlings, not only provides the basis for the later the scientific research, and provide technical guidance for production. Through the experiment, the results show that:1.The in vivo materials best sterilization methods:Actinida polygama:ethanol15 s + 0.1% mercuric chloride 7 min disinfection best; ‘Qihong’ kiwi:ethanol15 s + 0.1% mercuric chloride :6 min disinfection best; ‘Qinmei’ Kiwi:ethanol 15 s + 0.1% mercuric chloride 8 min disinfection best.2.The in vivo materials picked in Mid-April or Mid-May are the best for induction and sterilization, with the highest survival rate(87.6 %), the lowest stained rate(8.67 %).3. The medium for the first propagation:Actinida polygama、‘Qihong’ kiwi、‘Qinmei’ Kiwi MS+4.0 mg/L 6-BA + 0.34mg/L NAA the best combination of medium formula. The medium for the subsequent propagation: MS+4.0mg/L 6-BA +0.34mg/L NAA the best combination of medium formula. The medium for inducing roots: 1/2MS+ IBA0.6 mg/L+IAA 0.3 mg /L the best combination of medium formula.4. When hardening transplanting, transplanting the best matrix ratio: 1/3 vermiculite: 2/3(sand: Mountain clay) =1:1:2, the survival rate was 93.3%.