Study On The Regulation Of Astragalus Polysaccharide Supplementation In Diet Of Male Chicken Breeders On Intestinal Tract Function Of Male Chicken Breeders And F1 Broilers

The intensive poultry production is harmful to broiler immunity. Immunological stresses of broilers caused by stressors give rise to decreasing of growth performance and broiler industry profit, especially in the condition of antibiotic restriction. Therefore, we need to find a new alternative additive of antibiotic urgently. Astragalus polysaccharide(APS) is a kind of polysaccharide substance which possesses a wide range of bioactivities such as anti-inflammatory, antibacterial and growth-promoting. The researches in nutri-epigenetics have indicated that some nutrients could modulate the traits of their offsprings through epigenetic modification. In this study, we supplemented APS into male chicken breeder’s diet and detected the effects of paternal regulation and provided foundation for the study about patriarchal transfer regulation.Exp.1 Effects of APS supplementation in diet of male chicken breeders on intestinal morphology of male chicken breeders and F1 broilersTotal 160 healthy Cobb 500 male chicken breeders were divided into 5 groups randomly,4 replicates per group and 8 male chicken breeders per replicate. Male chicken breeders were fed diet with 5 different APS levels(0, 0.001%, 0.01%, 0.1% and 1% in the basal diet).Female chicken breeders were fed basal diet and breeder’s eggs were hatched. After incubating, F1 broilers were fed basal diet. The results showed that addition of APS had no effects on body weight gain of 32-week-age male chicken breeders. The addition of 1%APS decreased duodenum crypt depth of male chicken breeders(P<0.05), and increased jejunum villus height and V/C of male chicken breeders(P<0.05). Addition of APS in diet of male chicken breeders decreased jejunum crypt depth of F1 broilers(P<0.05). Addition of 1%APS in diet of male chicken breeders increased V/C of F1 broilers(P<0.05) and the same as male chicken breeders. The present results show that, the 1%APS supplementation improved intestinal morphology of male chicken breeders; the 1%APS supplementation in diet of male chicken breeders improved intestinal morphology of F1 broilers.Exp. 2 Effects of APS supplementation in diet of male chicken breeders on intestinal transporters and intestinal mucosal integrity of male chicken breeders and F1 broilersOn the basis of last experiment, the intestinal mucosal samples of male chicken breedersand F1 broilers were collected on days 14 after broilers birth. The sIgA content was determined using ELISA kit, the relative mRNA expression of glucose and amino acid transporters(SGLT1, GLUT2, rBAT and y+LAT2), tight junctions(ZO-2 and Occludin),factors related to intestine development(PCNA and TGF-β), key factors of TLR4 signaling pathway(TLR4, MyD88 and NF-κB) and cytokine(IL-4 and TNF-α) was determined using real-time quantitative PCR(RT-qPCR). The results showed that addition of 1%APS had no effects on the expression of these factors or sIgA content in male chicken breeders. Addition of 1%APS in diet of male chicken breeders up-regulated the expression of ZO-2 in F1 broilers jejunum and ileum(P<0.05), up-regulated the expression of Occludin in F1 broilers ileum(P<0.05), up-regulated the expression of NF-κB in F1 broilers jejunum and ileum(P<0.05)but had no effects on the expression of other factors or sIgA content in F1 broilers intestine.The results of exp.2 show that, the 1%APS supplementation in diet of male chicken breeders up-regulate NF-κB and tight junctions expression, which have benefit for improving development of intestinal mucosa and maintaining intestinal structure integrity of F1 broilers.In summary, 1%APS supplementation in diet of male chicken breeders up-regulated NF-κB and tight junctions expression, which regulated intestinal musocal development,improved jejunum morphology and protected intestinal structural integrity of F1 broilers,finaly improved early-period growth performance of broilers.