Mapping Research Of TaSDA1 Gene Of Triticum Aestivum Spike Development Atrophy1

Wheat(Triticum astivum L.) as one of the world’s most important food crop, the production level is one of the most important problems of the global food security. In the modern times, with the increased population and improved level of consumption, there is also a high requirement of wheat yield potential. The development of the three yield constituents such as the ear number, spike number per unit area and TKW( thousand kernel weight) all depend on the development of the wheat spikes. So the research of the spike development related gene will do significant meaning to the genetic improvement of wheat yield traits. Until to now far, studies on wheat mutants always obtained by physical and chemical mutagenesis, and mutation orientation results unstable and there is little reports on the research of wheat genes related to spike development. Using this wheat mutants to make study on the relationship between yield and panicle development genes, will make great important to the genetic mechanism research and lay a theoretical foundation to the molecular breeding. Materials used in this study is found in the breeding field, its one gene of Triticum Aestivum spike development atrophy1 mutant and showed narrow leaf, small height and without fruitage. The research of gene mapping of Ta SDA1 including: investigating and analysis the main agronomic traits of SDA1 and wild-type; using 2-DE to research the different expressed proteins of SDA1 and wild-type; and using shaan 94 and the mutants hybrid get F2 population the hybrids get the F2 population to do gene mapping. The results were as following.1. Making researches about the main agronomic traits of wheat height, headin time, the length and width of flag leaf, spike number of SDA1 and wild-type SDA1, and using t test to deal with the results, finally we found that compared to the wild-type, SDA1 showed significant narrower narrower width in flag leaf, shorter in height and fewer spikelet number, and also the heading stage is delayed, the whole plant develops very slow.2. Using SSR microsatellite markers, and by analysis F2 segregation population, we finally mapped Ta SDA1 gene on wheat chromosome 6BL, between markers barc136 and EST marker SWES 181, the genetic distances are 24.49 c M and 11.08 c M3. Using two-dimensional electrophoresis technology to detected the total proteins differences of SDA1 and wild-type material on wheat heading time, and 27 differentially-expressed protein spots were selected by PDQuest(version 8.0.1) image software, among which 23 protein spots were successfully identified, compared with the wild-type, there were 6 proteins performanced missing, 17 proteins were down-expressed. These proteins involved in phorosynthesis, the dfference of resistance to oxidative The mutation phenotype of SDA1, might be related to the flag leaves’ differentially-expressed proteins in the decline of the photosynthetic capacity, disorder of the energy metabolism, the decline of oxygen stress resistance and coercive capabilities, process of m RNA editing and the blocked protein biosynthesis. It proves that the genes do pleiotropic effects.4. qRT-PCR was used to validate ribulose-1,5-bisphosphatecarboxylase/oxygenase large subunit(chloroplast)(SSP2) chloroplast-localized Ptr Tox A-binding protein1(SSP6), translation elongation factor G(SSP15) iosephosphate isomerase(SSP5209) gene expressions at the transcriptional evel, which were consistent with the protein expressed amounts detected by 2-DE.