Screening For Regulatory Factors Of Pol CMS Restorer Gene And GWAS Of Temperature-sensitive Genes In Brassica Napus

pol cytoplasmic male sterility(CMS) system is considered as ?the first CMS system of rapeseed with practical value?,about 60% seed production was used in China for commercial hybrid; pol TCMS is sterile at high temperatures and partially fertile at low temperatures, It also can support two lines. In order to fully understand the regulation mechanism of restore gene and temperature sensitive genes in pol TCMS, make more extensive use of heterosis, In this research we screening regulatory factors about Rfp by yeast one-hybrid, using genome-wide association analysis method to early positioning for temperature sensitive genes,The main findings are follows:1. By predicting of promoter analysis software for Rfp gene 600 bp upstream of the promoter sequence, the promoters contain the conserved domains of TATA-box and CAAT-box, and large numbers of light and stress-inducible cis-acting elements, Five vectors were constructed by 5?end deletion were transformed into Arabidopsis, results showed that 151 bp were enough to conferring the GUS expression,118 bp did not conferring the gene expression. we constructed a cDNA library with a bud(0.5-1mm) of Brassica napus restorer lines construction of yeast bait vector with gene 394 bp upstream of the promoter sequence, we capture a protein interacted with Promoter from cDNA library of Brassica napus by yeast one-hybrid. through bioinformatic analysis revealed that the gene may be involved in cell stress biology, other defense mechanisms2. 86 Brassica napus maintainer lines from 530 parts of natural populations with 6330 A construct F1 hybrids as groups, the hybrid populations were sown in the fall of Wuhan This groups was partially fertile at low temperatures(less than 16℃)early March, while all infertility in high temperatures. Explaned that temperature-related gene in this group. Use 60 K SNP Chip Information combined with F1 groups phenotypic data, use Q models detected that there are a plenty of marker loci in A9、A5、A2、A8 chr may associated temperature sensitive variation.