Cloning Of MAX2 Homologous Genes In Populus

Branching is a very complex physical development processe to plants, it involves many physiological mechanisms, the effect of plant hormones on branching is one important aspect. In 2008, a new plant hormone is been discovered--strigolactone, of which the function is to participate in regulating the branching of aboveground. Currently, it has made a progress on the mechanism of strigalactone’s regulating branches. Many strigolactone-related gene have been cloned, including Arabidopsis thaliana,Oryza sativa, Pisum sativum L., petunia hybrida.But at present, the specific ways of synthesis and signal transduction of strigalactone still not clear, in different species have been cloned limited genes, particularly for its role in the mechanism of woody plants still in its infancy. Populus, as the model plant in woody plants,explore the related molecular mechanism of strigalactone’s influence is of great importance.In this study, with the bioinformatics analysis, we found and select the homologous genes of SLs in Populus trichocarpa. Through the analysis of the expression and function of each gene, choosing the the homologous genes of MAX2 of SLs’signal pathway to clone. At present, the experiment used the bioinformatics methods to select the target gene,has been made the relevant expression analysis.By the Gateway technology, we cloned the homologous genes of AtMAX2(AT2G42620) in Arabidopsis thaliana,and constructed the overexpression vectors in Col-0 Arabidopsis thaliana;also.Simultaneously, used the Populus tremula ×P.alba INRA clone N717 1-B4 for the plant material, overexpressed the AtMAX2 in N717. It has been obtained the transgenetic seedling of overexpression of AtMAX2 in N717.We cloned the homologous gene Potri.006G068500 of PtrMAX2c.Overexpressed AtMAX2 to N717, and it has identified positive plants.We cloned the homologous gene Potri.006G068500 of PtrMAX2c in Populus, and constructed the overexpression vectors in Col-0 Arabidopsis thaliana. It has been obtained the seeds of PtrMAX2c transgene in AT_T0. After three generations’ selecting and RT-PCR identification of Arabidopsis thaliana plants, it has been obtained 9 overespression AtMAX2 of Transgenic Arabidopsis thaliana homozygous.After positive plants selecting and RT-PCR identification, it has been obtained 3 overespression PtrMAX2c of Transgenic Populus trichocarpa homozygous.