Quantitative Methods Confirmed Swine Four Organizations Baclofen In Pakistan

As people on safety and quality of food of animal origin have become increasingly demanding, health and environmental protection farming will become a trend in recent years, irrational drug use in food animals in the breeding process and illegally added illicit drugs, as well as non-compliance with withdrawal regulations and so on, so that these problems endanger food gradually increase, which we engaged in food research and testing relevant industry researchers put forward higher requirements, how fast, sensitive and efficient detection of veterinary drug residues in question be placed in us The primary challenges ahead. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a food safety one of the main techniques of drug residue analysis, the non-volatile, thermally unstable, identification and separation of high boiling compounds has a unique advantage, relative to the substrate complex The sample has a high sensitivity, high selectivity and other characteristics.Baclofen by an chlorophenyl and a carboxyl group and an amino group composition, and therefore have amphoteric, soluble in water and other acute organic solvents. Baclofen is β-agonist, and banned the use of additives used, because of its nutritional redistributive effect, can effectively improve the lean meat yield, and therefore can be used as an alternative clenbuterol which product is used for animal breeding, it is necessary to develop methods to detect residues, to prevent criminals use in animal breeding. However, some methods have been reported for baclofen in the animal’s urine and muscle of residue analysis. So, the purpose of this work is to establish a relatively sensitive LC-MS/MS confirmation by detection of porcine liver, kidney, muscle, fat content remains in baclofen. The method used is after acid hydrolysis, the sample with isopropyl alcohol-extracted with ethyl acetate (4:6), evaporated after reconstitution through SPE and collected, was finally collected and evaporated to dryness and re-dissolved in water/methanol mixture by LC-MS/MS was measured. Using selected reaction monitoring (SRM) positive ion detection baclofen. The method of liver, kidney quantitative, muscle and fat limits were 1.0,1.0,1.0 and 1.0 ug/kg. The method according to the European Commission 2002/657/EC resolution linearity, accuracy, precision, specificity, CCa, CCβ verified. All the correct value is maintained at between 90.45% and 92.8%. The accuracy of the test concentration levels of value with excellent relative standard deviation (RSD<15%). The method CCa and CCβ values of each organization has been established. This method is suitable for detecting residues of baclofen in the four pigs in the organization.