Study On Bio-ecology And Its Relative Enzyme Activity In The Wolf Spider,Trchosa Ruricola

By using ultraviolet spectroscopy and acid base titratim, a comparation study was made on the digestive enzyme activity (Lapase and Proteinase) of the spider, Trchosa ruricola during overwin-tering and non-overwintering.The result dedicated that the digestive enzyme activity in the spider during non-overwintering was higher than that during overwintering.The result from the differentidal centrifug-ation indicated that the major portion of digestive enzyme located in the cycosol fraction.These results suggested that digestive enzyme existed in the cell of the spider mainly as the form of soluble protein molecule. This paper studies also the temperature to be T. Ruricola Body Acetylcholinesterase distribution. The results showed that:At 4 ℃ the activity of AChE in any part of the T. Ruricola body [including cephalothorax with the corresponding value2.826nmol/(mg·min)、abdomen2.564 nmol/(mg·min)and appendages 1.054 nmol/(mg·min)] to be lower than at room temperature the activity of AchE in any part of the T. Ruricola body [including cephalothorax with the corresponding value 3.257 nmol/(mg·min), abdomen 3.104 nmol/(mg·min) and appendages 1.082 nmol/(mg·min)], That at low temperatures to be spider Pardosa decreased metabolism. To be spider Pardosa AChE in vivo mainly concentrated in the cephalothorax, the distribution to adapt their physiological characteristics.Acetylcholinestrase(AChE) was purified from T. Ruricola by PEG2000/phosohate-salt two phases extraction and DEAE Spehadex A-50.The result of purified tested by spectroscopy.The result suggested that after DEAE Spehadex A-50,the specific activity of enzyme was twice higher as before.