Induced Resistance To Soybean Root Diseases By Seed-coated With Microbial Fermentation Broth

The production of soybean is serious influenced by the soybean cyst nematode (Heterodera glycines, SCN) and root rot diseases by Fusarium spp.. As the targets of SCN and Fusarium sp., the microbial strains were screened by soybean seed treatment to induce the soybean to produce the resistance to the pathogens. The strains with high induced activity were identified. The induced resistance mechanism and other aspects of the systems were studied.1. Screening strains induce soybean resistance soybean cyst nematode and FusariumSoybean cyst nematode and soybean root rot Fusarium were as the targets. The 4650 fermentation broth coated soybean seeds in the field after the initial screening. The following year and then by greenhouse and field screening, the eight efficient strains were obtained, include Snef805, Snef1650, Sneb572, Sneb877, Sneb1076, Sneb1401 and Sneb1499. The results showed that the efficient strains, Snef805, Snef1650, Sneb572 and Sneb1076 could not only induce the resistance to soybean root rot Fusarium but also to soybean cyst nematode in greenhouse and field trials.The soybean treated was promoted in growth and increased for production.2. Identification of Efficient Soybean StrainsOn the basis of morphological, physiological and biochemical reactions, through molecular biological methods and systems for efficient Biolog, the strains Snef805, Snef1650, Sneb572 and Sneb1076 were classified and identified. The Snefl650 is Penicillium janthinellum, the Snef805 is Penicillium chrysogenum, the Sneb572 is Enterobacter cloacae, and the Sneb1076 is Bacillus thuringiensis.3. Biocontrol activity of Penicillium janthinellum Snef1650 and its effect on crop growthThe peanuts and green beans treated by the broth of Penicillium janthinellum Snef1650 were promoted on the growth, two species of nematodes (H.glycines, Ditylenchus destructor) with the virulence effect.4. Efficient soybean strains induced systemic resistance (ISR)Crack root test results showed that the activity of the strain broth after fermentation process produced the induced systemic resistance to soybean cyst nematode, Fusarium oxysporum and Fusarium solani in the root.