Molecular Genetic Map Construction And QTL Mapping For Flavonoid In Hawthorn (Crataegus Spp.) Leaves

In this study, an F1 mapping population consisting of 92 hybrid individuals was created using ’damianqiu’(female parent) and ’qiujinxing’(male parent). Hawthorn molecular genetic map was constructed by SRAP and SSR molecular markers. Flavonoid component in the leaf of hawthorn were extracted by ultrasonic-assisted extraction and quantitatively analyzed by High Performance Liquid Chromatography (HPLC). QTLs of five flavonoid monosomics were mapped. The main results were as follows:1、240 SRAP primer pairs were screened, among which 47 pairs produced clear and polymorphic bands; 322 SSR primer pairs were screened by annealing temperature and polymorphism, gainning 84 polymorphic SSR primers.2、Authentic identification was done for the hybrids of ’damianqiu’ ×’qiujinxing’ using one paternal special SSR marker ’8UB13155-188’. The true hybrids were verified.3、All these 47 SRAP primer pairs and 84 SSR primer pairs were used for the amplification of mapping population, generating 71 maternal special markers,103 paternal special markers and 225 common markers. Genetic maps of both parents were constructed by software JoinMap3.0 and software Mapchart2.2. The maternal map contained 153 markers allocating into 21 linkage groups, and it covered a total length of 1085.4 cM, with a mean distance between adjacent markers of 7.05 cM. The paternal map contained 170 markers allocating into 22 linkage groups, and it covered a total length of 1097.7 cM, with a mean distance between adjacent markers of 7.73 cM.4、QTL detection of five flavonoid was done by MapQTL5.0 software and using the Multiple-QTL model (MQM). In maternal map,14 and 18 QTLs of flavonoid component (rhamnosylvitexin, vitexin, rutin, hyperoside and quercetin) were detected in the year 2014 and 2015 respectively. They can explain the rate of contribution from 17.2% to 76.6%. In paternal map,10 and 19 QTLs of flavonoid component (vitexin, rutin, hyperoside and quercetin) were detected in the year 2014 and 2015 respectively. They can explain the rate of contribution from 26.5% to 71.49%.The genetic maps for hawthorn ware successfully constructed and the QTL of flavonoid for hawthorn was targeted. The present research lays the foundation for hawthorn marker assisted selection and flavonoid gene cloning, and for genetic machanism study of hawthorn flavonoid, there were important significance.