| Bacterial stripe of rice and bacterial blight are two of the most common and important bacterial diseases. They are widely distributed in the rice-growing area and cause heavy economic losses. Nowadays, chemicals are generally used to control these diseases. However, their frequent uses have resulted in the emergence of drug-resistant pathogens and environmental problems. Therefore, there is an increasing need to develop new alternative strategies to control the outbreak of these bacteria-caused diseases. Phages were proposed to be an attractive alternative to pesticides and this biological therapy has some unique advantages including high host specificity, self replication and low environmental impact. In this study, phages specific to Acidovorax avenae subsp. avenae and Xanthomonas oryzae pv. oryzae were isolated based on screening of 27 A. avenae subsp. avenae strains and 23 X. oryzae pv. oryzae strains and their characteristics were determined.In addition, the study detected the impact of phage on morphology, motility, EPS production, biofilm formatiion of A. avenae subsp. avenae.Phage adsorption specificity associated with phage receptor was investigated and LPS was determined to be a receptor in this study. Furthermore,RlpB genes were revealed to be related to LPS synthesis through the A. avenae subsp. avenae genome analysis.RlpB mutant was obtained using the homologous recombination strategy.Through comparison with the RlpB mutant strains, it showed that the capacity on adsorption of phage to wild-type strains decreased significantly,indicating that RlpB gene may play an important role in the process of adsorption.Further researches on the effect of bio-control of phage on bacterial stripe of rice and bacterial blight showed a good effect on bio-control of phage at the early stage of infection. In a conclusion, the result of this study indicated that the phages have a great potential in biocontrol of the two main bacterial diseases in rice. |
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