The Analysis And Identification Of Palmitoylated Proteins In Response To The Interaction Between Influenza Virus And Host Cells

Avian influenza is an avian infection syndrome caused by avian influenza A virus and highly pathogenic avian influenza(HPAI) can cause high mortality in poultry. Because the virus can spread across species, human can be one of the hosts of avian influenza virus. Avian influenza has caused great threats and loss to human health and social economy. It is one of the viral infectious disease in poultry industry in recent years and is identified as one of the most serious infectious diseases by both the Office International Des Epizooties and China goverment.In this study, we used African green monkey kidney cells(Vero cells) to propagate avian influenza virus(H5N1), followed byacyl-biotinyl exchange treatment. Mass spectrometry-based proteomics strategies was used to analyze the palmitoylated proteins in response to the interaction between influenza virus and Vero cells. The results from proteomics research are as follows:(1) In blank control sample, 266 candidate proteins were identified,including 56 palmitoylated proteins with high confidence.(2) In Vero cells infected with avian influenza virus, 172 candidate proteins were identified, including 28 palmitoylated proteins with high confidence.Because the palmitoylation modification was mainly found in the membrane proteins and membrane associated proteins, researchers usually use the membrane fraction as the source to study the palmitoylated proteins. Whereas the palmitoylated proteins in the cytosolic fraction are seldom studied due to their extremely low concentration. In the present study, we performed the further study on the changes of palmitoylation modification of cytoplasmic proteins in the Vero cells in response to influenza virus infection. The results are as follows:(3) In blank control sample, 40 candidate proteins were identified, including 5palmitoylated proteins with high confidence.(4) In Vero cells infected with avian influenza virus, 56 candidate proteins were identified, including 17 palmitoylated proteins with high confidence.