| Powdery mildew, caused by Blumeria graminis f. sp. tritici(Bgt), is one of the three most devastating diseases in wheat and influences the yielding seriously. Breeding and using resistant cultivars is the most widely employed strategy to curb this disease. Discovering new powdery mildew resistance genes and their application in breeding is a constant task due to the co-evolution of pathogen virulence and host resistance. In addition, precise mapping and cloning of resistance genes will lead to an in-depth understanding of host resistance mechanism and a more effective molecular resistant strategy for continuous control of this disease.The recessive resistance gene pm2026, identified from the einkorn wheat accession TA2026, was mapped to the 0.2 cM Xmag5222-Xmag2170 interval on chromosome 5AmL in previous study.1131 individuals from TA2026×M389 F2 population were evaluated and genotyped by closely linked markers. In total,17 recombinants were obtained. Combining the data of 1131 F2 individuals and 846 resistant homozygous F2.3 families in previous study, pm2026 was precisely mapped to 0.08 cM interval.The recessive powdery mildew resistance gene pm412 was identified from the einkorn wheat accession 223412 and located in the 24 cM interval flanked by Xcfa2019 and Xmag600 on chromosome 7AmL. In this study,162 plants of 223412×M389 F2 population were evaluated and new markers were developed based on the colinearity of diploid, tetraploid and hexaploid wheat. Finally, the pm412 locus was mapped to a more precise 4.8 cM interval, flanked by Xwgrc104 and Xwgrcll 7 with 3.2 cM and 1.6 cM, respectively. |
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