Toxic Effects Of 3-Methyl-4-Nitrophenol On Liver And Reproductive And Attenua- Ting Effect Of Soy Isoflavone In Broilers

3-Methyl-4-nitrophenol (PNMC) and 4-nitrophenol (PNP) are two common nitro phenols, and they belong to environmental endocrine disruptors which widespreadly in the environment and have a certain lethality. Chicken is an indispensable food and security broiler feeding environment is directly related to our health. The present study is designed to elucidate the effect of PNMC on growth and development in chicken and its cytotoxicity. This study mainly includes three parts presented as follows:1. Effects of 3-methyl-4-nitrophenol on the liver and reproductive organs inbroilersIn the first part of this study, we added 10 mg/kg and 100 mg/kg PNMC in broiler’s drinking water to simulate contaminated drinkingwater, and investigated the effect of PNMC on growth performance,liver andreproductive organs.961-day-old AA broiler (half male and half female) randomly divided into 3 groups, boiler were fed in cages for 42 days.The results indicated that PNMCdidnot cause visual toxic symptom and behavioral abnormalities; PNMC (10 mg/kg BW) significantly reduced broiler performance; PNMC (10 mg/kg BW)induced vacuoles in liver tissue and seminiferous epithelium; Some leydig cells and spermatogenic cells displayedobvious apoptosis; PNMC (10 mg/kg BW) also disturbedthe endocrinesystem.2. Alleviative effect of soy isoflavone on 3-methyl-4-nitrophenol-inducedtoxic damagein liver and reproductive organs in broilersIn the second part of this study, we investigated whether soy isoflavone can relieve the effects caused by PNMC, via adding 100 mg/kg soy isoflavone in their diet.1281-day-old AA broiler(half male and half female) randomly divided into 3 groups, boiler were fed in cages for 42 days.The results indicated that both PNMCand dietary soy isoflavone didnot cause visual toxic symptom and behavioral abnormalities; PNMC (10 mg/kg BW) significantly reduced broiler performance while dietry soy isoflavone (100 mg/kg) couldameliorate this adverse effect; PNMC (10 mg/kg BW) also disturbedthe endocrine systerm while this effectwas attenuated by dietary soy isoflavones.3. Cytotoxicity of 3-methyl-4-nitrophenol and 4-nitrophenol on MCF-7, MCF-10A, NMuMG and 4T1cell linesWe subjected MCF-10A (hHuman breast epithelial cells), MCF-7 (human breast cancer cell), NmuMG (Mammary epithelial cells in mice) and 4T1 (Breast cancer cells in mice) to different concentrations of PNMC and PNP to determine its cytotoxicology, respectively. PNMC (10-4mol/L) especially depressed the growth of MCF-7 cells. PNMC has specific inhibition on the growth of MCF-7.In conclusion, PNMC impacted growth performance, and damaged liver and reproductive organs in broilers. Low concentration of PNMC induced more severe in hepatic injury among treatments; Dietary soy isoflavone could partly alleviate the adverse effects; PNMC has specific inhibition on the growth of MCF-7.