Effect Of NaCl On Growth And Physiology Of Seashore Paspalum And Zoysia Japonica

To explore the seashore paspalum(Paspalum vaginatum)’Seaspray’ zoysiagrass (Zoysia japonica) ’Lanyin NO.3’salt-tolerant mechanism for breeding salt-tolerant turf grass and green manufacturing process provides a theoretical basis to resist salt stress. A pot experiment was conducted to study the different NaCl concentrations (100,200,300,400 and 500mmol/L) treatment on seashore paspalum (Paspalum vaginatum) and Zoysia grass (Zoysia japonica) growth, physiology, photosynthesis and root physiological indicators, and by means of micro-root tube technology (Minirhizotron) on seashore paspalum and zoysia root growth under salt stress to the demise of the process of root length, root surface area, root projected area, root diameter, root volume-based rate of root growth into the in-situ monitoring the following conclusions.(1) Under NaCl treatment the growth of seashore paspalum and zoysia are affected. In the 14d and 21d under salt treatment, at the NaCl concentration of 300, 400 and 500 mmol/L the relative growth rate of two grass biomass and leaf relative water content are significantly lower then the CK. Compare the value under 500 mmol/L of three observations we can find that the relative growth rate and biomass of seashore paspalum and zoysia significantly reduce with stress time extend.(2) Different concentrations of NaCl treatment affect the physiological effects of seashore paspalum and zoysia.At three observation period(7d,14d,21d), with the increasing of NaCl concentration, Zoysia soluble protein content, relative electrical conductivity and MDA had a rising trend meanwhile at 21d under 400 and 500 mmol/L NaCl concentration, relative electrical conductivity, MDA and proline content and soluble protein content had a more significant rising trend than control. Compared the values at three observation period under 500mmol/L respectively, we found that relative growth rate, biomass and leaf relative water content of Zoysia were decreased and relative electrical conductivity, MDA and soluble protein were increased with a greater salt stress. Under high NaCl concentration, POD and CAT enzyme activity were low.(3) Different concentrations of NaCl treatment affect the photosynthesis effects of seashore paspalum and zoysia Pn was higher than control at 100mmol/L NaCl concentration, but other treatments Pn were decreased with NaCl concentration increasing.Gs and Tr were declined at high NaCl concentration and 500mmol/L treatment was significantly lower than control. WUE and Ls were increased with NaCl stress and significantly higher than control under 400mmol/L NaCl concentration at 7d.(4) The results showed that:Seashore Paspalum and Zoysia root length increased with increasing of salt stress at first and then decreased after reaching to the maximum at the 100 mmol/L NaCl concentration. Seashore paspalum root projected area and superficial area were significantly reduced at 300 mmol/L NaCl concentration, at same time Zoysia’s downtrend were began at 100 mmol/L NaCl concentration. Salt stress stimulated Seashore Paspalum root diameter increase and each treatment were significantly higher than the control. Zoysia root diameter were decreased because of salt stress inhibition.