Preparation Of Inactivated Vaccine With High Pathogenic Fowlpox Virus

Fowlpox is caused by Fowlpox virus, an acute and contact infectious disease,widely occurred in raising industry, all age and varieties are susceptible, but at the highest of the sensitivity of the chicks, clinically main performance for the skin,mucous membrane and hybrid. The disease have occurred throughout the world,the main use pigeon pox vaccine and chicken pox quail weak poison vaccine immunization to prevent. In recent years, due to the strain variation caused by FPV infection reported increased, catering industry caused great economic losses.Especially in 2009 in jilin province gongzhuling a large chicken pox epidemic such farms along the skin type, in 10000 only brown laying hens died all 46 within 10 days after onset of day age, for the effective prevention and control of the disease is a wake-up call again, at the same time also to existing part brought challenge to the validity of the commercialization of the vaccine. In order to further improve the chicken pox vaccine immune protection effect, this research to the current popular chicken pox virus as the kind of poison the inactivated vaccine of laboratory research work.The early stage of the team from jilin gongzhuling an outbreak of type skin chicken chicken pox epidemic died a highly pathogenic strains were isolated by chicken pox virus, named FPV JL 09 C2. By genome sequencing, found that the genomes of the FPV integration over nearly the reticular endothelial tissue hyperplasia of poultry virus(REV) gene. This study selects the FPV JL 09 C2 strain as a kind of poison, the highly pathogenic chicken pox virus inactivated vaccine research. Through the test to determine the strain on the chicken embryos best answer the toxic dose and toxic time, which USES 150 ul poison disease vaccination SPF chicken embryo culture after 5 d poison for optimum condition. In this condition to virus expanding training and selection of formaldehyde as an inactivating agent to inactivate the virus, 24 h can make sure 3‰ formaldehyde treatment completely inactivated virus. According to "regulations of veterinary biological products" compound aluminum adhesive adjuvant, and inactivated viruses 1:9 according to the proportion of mixture, the preparation of a good vaccine bacteria inspection according to the national standard, using PCR amplification technology to mycoplasma test. According to the production process preparation of chicken pox virus inactivated vaccines for follow-up studies.For inactivated vaccine developed for the determination of minimum immune dose and immune effect evaluation. The results showed that the vaccine immune to chickling minimum dose is 0.2 ml. Then for the preparation of vaccine safety inspection, the result shows that with a single dose, repeated a single dose and a super dose after inoculation of experimental animals, through clinical observation,temperature measurement and pathological observation clinical side effects, such as that the vaccine does not produce good security. ELISA tests, chicks 2 to 3 weeks after vaccination FPV antibody level has reached the highest, the highest OD450 can reach 0.55, but each REV antibody was detected in serum of existence;Lymphocyte proliferation test, compared with the PBS group, inactivated vaccine group of CD3+, CD4+ T lymphocyte percentage increases; The percentage of CD3+CD8+T lymphocytes has no obvious change; Inactivated vaccine immunization group and after specific antigen stimulation in highly active state; Of cytokines detection showed that the vaccine can stimulate the chicken to produce specific immune response, and mainly Th2 type of immune response. Through to the vaccinated chickens against poison, which can reach 85%, determination of shelf life of vaccine, the vaccine under 4 ℃ and room temperature environment save 9 and 3 months respectively, its physical properties and immune effect did not change, this is the same as inactivated vaccine characteristics.Thus, this laboratory preparation of FPV inactivated vaccines can effectivelystimulate the body’s immune response, so as to achieve the purpose of prevention of the disease. The development of the vaccine for chicken pox effective prevention is of great significance.