The Construction Of DNA Fingerprinting In Jute Genetic Resources

Jute, one kind of the most important long bast fiber annual herbaceous crop, belongs to the genus Corchorus classified under the family Tiliaceae. It includes about 40 species, among them round fruit jute (C. capsularis) and long fruit jute (C. olitorius) are the common cultivars. At present, molecular marker technology is widely applied in jute studies, such as the origin and evolution, genetic diversity, and genetic map, but rare reports on genomic DNA fingerprint construction due to self-pollination, unsignificant genetic differences, and high genetic similarity in the jute. The construction of DNA fingerprint will provide the jute with effective technical support in genetic relationship and taxonomic study, purity identification and authenticity test, the identification of germplasm resources, the establishment of core collection, varieties protection, and the registration of new varieties.This study, containing 231 jute varieties, conducted the construction study of DNA fingerprint for jute varieties, with three kinds of molecular markers, SRAP, ISSR and SSR, and DNA fingerprint analysis software, combined. The main results are summarized as follows:1.35 pairs of SRAP primer combinations were selected to investigate the DNA maker of 231 jute varieties, and the DNA fingerprints were gained from 96 varieties. The average contribution rate of these SRAP primers is 13.6. Every jute variety was equally identified by 3 specific primers, some varieties were identified by 34 SRAP primers, but the rest could be identified by none of the primers, so the conclusion is that there are differences in genetic background among these jute varieties.2.11 polymorphic ISSR primers were selected to investigate the DNA maker of the selected jute varieties, and the DNA fingerprints were gained from 45 varieties. The average contribution rate of these ISSR primers is 53. Every jute variety was equally identified by 7.2 primers, some varieties were identified by 11 ISSR primers, and some were only identified by 2 primers.3.49 polymorphic SSR primers were selected to investigate the DNA maker of the selected jute varieties, and the DNA fingerprints were gained from 13 varieties. The DNA fingerprints of some varieties were mapped by using two primers, as well as some done three primers. The average contribution rate of these SSR primers is 27.6. Every jute variety was equally identified by 26.8 primers, some varieties were identified by 49 primers, but the rest couldn’t be identified by any one of the selected primers.4. Through analysis of three molecular marker methods, SRAP, ISSR and SSR, on the selected jute materials, the DNA fingerprints of 154 jute varieties were constructed. Yueyuanqing, Yueyuan 5, Tianma were found to be the only three ones that could be marked and mapped by these three molecular marker methods. The results indicated that a same variety produced different DNA fingerprints according to different molecular markers, every identified jute variety has its unique "ID".