The Antimicrobial Resistant Genes Detected Of Intestinal Lactic Acid Bacteria Isolated From Forest Musk Deer By PCR And Development Of Compound Probiotic For Forest Musk Deer

This study investigated antimicrobial resistant genes of intestinal lactic acid bacteria isolated from forest musk deer for providing a resistant reference of selecting strains from intestinal lactic acid bacteria isolated from forest musk deer as compound probiotics and for monitoring antimicrobial resistance of intestinal lactic acid bacteria of forest musk deer. Developed an effective compound probiotics using lactic acid bacteria strains which isolated from gut of forest musk deer, it is a preliminary study on forest musk deer using compound probiotics in clinic.(1) 26 antimicrobial resistant genes which were sulfanilamides (sul1, sul2, sul3), quinolones (qnrA, qnrB, qnrS, qepA, aac(6’)-Ib-cr), tetracyclines (tetA, tetB, tetC, tetD, tetE, tetM, tetW), aminoglycosides (aph(3’)-Ⅱa, ant(3")-la, ant(6)), β-lactam (blaTEM, blaSHV, blaCTX-M, bla), vancomycin (vanA, vanB), and Chloramphenicols (cat, catA) were detected in 14 intestinal lactic acid bacteria isolated from forest musk deer using polymerase chain reaction (PCR). The results showed that sulfanilamide(sul1, sul2, sul3), quinolones (qnrA, qnrB, qnrS, qepA), tetracyclines (tetA, tetB, tetC, tetD, tetE, tetW), aminoglycosides (aph(3’)-Ⅱa, ant(3)-Ⅰa, ant(6)), β-lactam (blaTEM, blaSHV, blaCTX-M, bla), vancomycin (vanA, vanB), Chloramphenicols (cat, cat A) were negative. While, there were 2 positive detected results of antimicrobial resistant genes, including aac(6’)-Ib-cr (57.14%) and tetM (57.14%).(2) This study designed three different compound probiotics were fed for mice, the data showed that the amount of lactic acid bacteria in cecum of mice of group C more than the other two groups. Then this study chosen group C (Lactobacillus plantarum: Lactobacillus acidipiscis:Leuconostoc fallax=1:1:2) for following trails. The results of safety tests for compound probiotics C showed that the compound probiotics has no mar for body of mice, indicating that this compound probiotics is safe. Monitored the amount of lactic acid bacteria in compound probiotics, we found that the storage period is 14 d, and the optimum storage temperature is -20℃. After using compound probiotics C treated mice which infected by intestine pathogenic Escherichia coli in forest musk deer, the data showed that the compound probiotics group was significantly larger than the control group and the infected group (P<0.05), which revealed the compound probiotics could effectively control the proliferation of intestinal pathogenic E. coli in forest musk deer in intestine. The clinical cure rate of diarrhea of forest musk deer was 50%(3/6), the result showed that compound probiotics could have an effective influence on diarrhea of forest musk deer.