Genetics Analysis And Molecular Tagging Of Stripe Rust Resistance Gene In Sichuan Landrace Wheat Yilongtuomai

Stripe rust of wheat caused by Pucciniastriiformis f. sp. Tritici (PST), it spreads through the air and poses a serious threat to wheat productionworldwide. Growing resistant cultivars is the most environment friendly, economic and effective method to control wheat stripe rusts. From the point of current situation, antigen single situation hasnt changed in wheat production and breeding of China. Once the new physiological races emergence and popular, it will make a huge bad impact on production. So it is a great significance for digging resistance genes from different materials and applying the genes to breeding. Sichuan wheat cultivars grow in Sichuan where the wheat stripe rust epidemic, they are synergy evolution with the strip rust, and they contain rich of yellow rust resistance genes. This article has conducted the research to the Sichuan wheat resistant material Yilongtuomai. The main results are summarized as follows:1、Inoculated mix prevalent Chinese Pst races to Sichuan wheat cultivar Yilongtuomai and its progeny F1, F2 and F2:3 in field; and genetic analysis for the F1, F2 and F2:3 populations from a cross between Yilongtuomai and susceptible line Taichung29 show that, Sichuan wheat cultivar Yilongtuomai is resistant to all prevalent Chinese Pst races at all growth stages, and the resistance was conferred by a single dominant gene, temporarily designated as Yryltm.2、Using the bulked segregation analysis (BSA) method selected the primers about SSR, RGAP, TRAP, SRAP, et al. Further, using the primers tested F2 populations. Finally, the markers Xbarcl26、Xbarc92、Xswm10、cssfrl、cssfr2、cssfr5、csLV34、Xbarc352、 Xwmc438、Xcfd66、XLRR-INV1/CLRR-INV2、F-me4/R-me16、PtoFen-AS/AS1-INV, CLRRfor/Ptokin4 were found to linkage to Yryltm. Further the markers order and genetic distance was found by the linkage analysis software Mapmaker3.0. The markers order is Xbarc126-XLRR-INV1/CLRR-INV2-F-me4IR-me16-PtoFen-AS/AS1-INV- CLRRfor/Ptokin4-Yryltm-Xbarc92-Xswm10-cssfr5(Yr18)-Xbarc352-Xwmc438-Xcfd66. The flanking markers are RGAP marker CLRRfor/Ptokin4 and SSR marker Xbarc92, and the genetic distance to Yryltm is 5.35cM and 9.86cM respectively. Finally, the genetic map was obtained by the software Mapdraw.3、SSR marker and nulli tetrasome analysis placed Yryltm on chromosome 7DS.4、 Yilongtuomai and its F2 populotions were tested using functional markers of Yr18 including cssfr1、cssfr2、cssfr3、cssfr4、cssfr5 and STS maker CSLV34. The results show that Yilongtuomaimay contain Yr18 and Yryltm simultaneously. Combined with field resistant phenotypes, chromosomal location and molecular markers analysis, the relationsstill needs further research about Yryltm and Yr18 in Yilongtuomai.