| Objective:To provide a reference for prevention and treatment of mastitis in dairy cows, this paper explored the effect of different levels of granulocyte colony-stimulating factor (G-CSF) on mastitis of peripartum dairy cow.Method:1.100 of healthy heifers were selected. According to the level of G-CSF in serum before 7th day calving(-7d), the heifers were divided into low concentration of G-CSF group(Group I) and high concentration of G-CSF group (Group II),50 in every group.①Monitoring the mastitis in 1 month after calving, the milk simples of mastitis cases were collected and to identify the bacteria. ② Monitoring the daily milk production and detecting the milk ingredients at 7d、14d、21d、28d after calving within one month; ③10 mL blood of tail vein was collected before calving(-3d、-1d)、 calving day(0d)、after calving(1d、3d、7d) of heifers. 1mL of the EDTA anticoagulant blood for the detection of white blood cell count, and the remained 9 mL would be separated serum by centrifuged in 3000 r.m-1,-70℃ saved. Selecting the heifers whose calving time was consistent with expected date of confinement and without any disease within one month after calving,6 in each groups, measured the IL-4、IL-6、 IL-8、IL-10、IL-18、TNF-α in serum.2.100 of healthy multiparity cows were selected. According to the level of G-CSF in serum before 7th day calving(-7d), the cows were divided into low concentration of G-CSF group(Group I) and high concentration of G-CSF group (Group II),50 in each group. The test method was same with the heifers.Results:1 The heifers:①The mastitis incidence of group Ⅰ was 48%, the mastitis incidence of group II was 18%, the difference between the two groups were significant(P<0.05);② The milk productions in both groups were gradually increasing. The milk fat at 7d、21d and milk density at 14d of heifers in group Ⅱ were significantly higher than group I (P<0.05). ③WBC、GRA、LYM in group Ⅰ gradually decreased when approaching calving, and had not yet returned to normal one week after calving. IL-8、IL-18 increased approaching calving and decreased after calving; IL-4、IL-10、TNF-α decreased when approaching calving and increased after calving. IL-6 showed upward trend before and after calving. However, the WBC、GRA、MID、 LYM in group Ⅱ were significantly higher than those in group Ⅰ, and the cytokines above-mentioned were inhibited to varying degrees.2. The multiparity cows:① The mastitis incidence of group Ⅰ was 58%, the mastitis incidence of group Ⅱ was 16%, the difference between the two groups were significant(P<0.05);② The milk productions in both groups were gradually increasing. The milk compositions had no significantly difference between the groups(P>0.05).③ When approaching calving, WBC、GRA、LYM in group Ⅰ gradually decreased, IL-6、IL-8、IL-18、TNF-α were increased and IL-10 decreased. WBC、GRA、LYM in group Ⅰ were continued declining after calving, IL-4、IL-6、IL-10、TNF-α showed increased trend after calving. WBC、GRA、MID、LYM in group Ⅱ were significantly higher than those in group Ⅰ. IL-4、IL-8、IL-10、IL-18 in group Ⅱ showed an increasing trend and IL-6、 TNF-α showed decreased trend when approaching calving; IL-4、IL-10 showed an increasing trend and IL-6、IL-8、TNF-α showed a decreased trend after calving.Conclusion:1 The concentration range of G-CSF of heifers before calving was 327.77-648.89 ng.L-1. The high level of G-CSF before calving could significantly improve the number of white blood cell before and after calving, inhibit the change of cytokines before and after calving, and reduce the appearance of mastitis in perinatal period.2 The concentration range of G-CSF of multiparous cows before calving was 264.06-767.51 ng.L-1. The high level of G-CSF before calving could significantly improve the number of white blood cell before and after calving, improve the level of IL-4、IL-10 after calving, improve the ability of anti-infection, and reduce the appearance of mastitis in perinatal period. |
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