Studies On Mice Reproductive Toxicity Of Sanguinarine

Being a benzophenanthridine isoquinoline alkaloid, Sanguinarine (SA) has antibacterial, anti-inflammatory, anti-tumor, insecticidal and inducing apoptosis effects, and has been used as a new drug for medical and agricultural production. As a veterinary drug commonly used in the treatment of diarrhea, it has not been reported whether SA has security risks in the application in treatment with breeding domestic animals. The reproductive toxicity of SA on mice was systematically studied in this paper, in order to provide theoretical basis of clinical safe application of SA. The main contents and results are as follows:1. Cell proliferation inhibition test Porcine kidney epithelial cells (PK-15) and porcine testicular cells (ST) were treated with SA, and the number of cells were determined by MTS assay. Results showed that:.0.25 μmol/L could significantly inhibite ST’s proliferation, and 0.5 μmol/L SA can significantly inhibite PK-15’s proliferation. It prompted that SA may had male reproductive toxicity and nephrotoxicity.2. Male reproductive toxicity test 120 3-week-old male ICR mice were randomly divided into five groups:control group, the solution control group, low-dose group, middle dose group and high dose group, every mouse was gavaged 0.1 mL/lOg body weight per day continuously for 37 days, and the samples were collected and determined in the 24th,31st and 37th day respectively. The results showed the body weights of mice, the index of testis and epididymis, sperm count, sperm deformity rate, the organizational structure of the testis and epididymis, SOD, GSH-PX and MDA in testis tissue had no significant changes after treated by SA. It prompted that SA had no significant male reproductive toxicity.3. Embryo toxicity test in vitro ICR mice 2-cell embryos were cultured in vitro after treated by 0,1,2,4 μmol/L SA respectively, and the blastocyst hatching rate was calculated, blastocyst quality was observed, and the expression of Sox-2, Nanog and transcription factor Oct-4 were determined with real time RT-PCR.results showed that blastocyst hatching rate has certainly increased, but the blastocyst quality of SA treated group decreased, and the expression of transcription factor Oct-4 was inhibited to a certain extent, but the difference was not significant. It prompted that SA had no significant toxicity to embryos in vitro.Conclusion:SA has significant proliferation inhibition on PK-15 and ST cell in vitro, but had no significant toxicity on male mice reproductive system and embryos in vitro. It was initially identified that SA had no significant reproductive toxicity in mice.