Cloning And Sequence Analysis Of Full-length Genome Of Nairobi Sheep Disease Virus In Ticks From Northeastern China

As is known to all, Tick is one parasite that could parasites animal body surface and is an important media for some contagious diseases spreading between human and animals. Tick on the classification belongs to Animalia Arthropoda, Arachnida, Mite subclass, Send mites, Ticks superfamily. Around the world have discovered 18 genera and 897 species, and China has found 10 genera, 119 species. Tick can cause a variety of important natural foci of the disease and beast comorbidities, such as Q fever, fever, forest encephalitis, hemorrhagic fever with thrombocytopenia syndrome, etc. Nairobi sheep Disease virus on the classification of virus families belong to the cloth. The main medium is a tick on the wider public hazards can cause acute hemorrhagic gastroenteritis, sheep and goats mortality rates as high as 90%. In 1910, the disease was first discovered near Nairobi in Kenya, and its pathogen determines Nairobi sheep disease virus in 1917. Nairobi sheep virus disease was found in 1994 reported for the first time. Nairobi sheep disease occurs mainly in East Africa Kenya, Uganda, Tanzania, Ethiopia and Somalia. At present, Nairobi sheep disease has not been reported in China and East Asia, therefore, identification and whole genome sequence analysis of Nairobi sheep disease virus in northeast China are great significance.Based on the current tick class distribution in northeast China is not clear. This study sets in Jian、Jingxin、Chunhua、Nanshan、Songlin of Jilin Province, Haerbin、Huma of Heilongjiang province,Dandong of Liaoning province, more than 10 provinces and cities for the tick class collection. By morphological identification, mainly found four tick species, one of the most abundant is long-horned qinghaiensis ticks longicornis(84.8%), followed by dermacentor silvarum(7.2%), dermacentor nuttalli(5.5%) and Ixodes persulcatus(2.5%). Through the analysis of the macro virus genomics, a total of 26411 article reads, further joining together to get the average length of 108 base 18997 overlapping sequence. Among them, animal virus families, the largest number, 65% of the total number of virus sequence(7505/11547), including: Parvoviridae, Flaviviridae, Bunyaviridae virus families, etc.; Plant virus accounted for 19%(2189/11547); Phage sequence was 16%(1853/11547). Further to cracking of ticks organization, this study adopted RT-PCR method again to Nairobi sheep virus(NSDV) verification, the results obtained Jian、Jingxin of Jilin province and Dandong of Liaoning province region tick both Nairobi sheep with the virus. By virus macro genomics data and analysis of NSDVs virus sequence both at home and abroad for reference, this study successfully cloned sheep Nairobi(NSDV) genome of the virus. The sequence with foreign NCBI has included the whole genome sequences of nucleotide homology comparison, gene sequence comparison showed that at the nucleotide level compared to other NSDVs homology between 75.1 ~ 89.6%, the deduced amino acid homology between 81.3 ~ 96.7%. Compared to other members of a species NSDVs(Dugbe Kupe, Hazara and Crimean Congo hemorrhagic fever viruses), homology between 37.5 and 68.6%.This research confirmed the existence of tick-borne Nairobi sheep disease virus in East Asia region for the first time. In addition, based on the above tick morphology identification of positive region, Haemaphysalis longicornis Neumann can carry Nairobi sheep disease virus for the first time. The research has laid a good foundation for pathogenic and vaccine research of Nairobi sheep disease virus.