Analysis Of Codon Usage On Bos Grunniens And Bos Taurus

The study of the genetic code is of far-reaching significance to the understanding of the nature of life and the rapid development of molecular biology, molecular genetics and other disciplines. In this study, softwares were used to study the correlation analysis of genome codon preference in yak, genome codon usage characteristics and influence factors in bovine, codon usage bias and cluster analysis on mitochondrial genes in bovinae animals. The results showed that:GC content and GC3 s content at the coding region of yak genome are respectively 0.53±0.08 and 0.59±0.16. It is more affected by base mutation in yak genome codon usage bias. A handful of genes are affected by the choice. Eighteen main preference codons and five high-frequency codons were selected in yak genome. Codons with greater than or equal to 2 and less than or equal to 0.5 are respectively 0, 0, 1, 8, 0, 20, 3, 10, 12 in the 1/1000 ratio of Bos grunniens, Bos Taurus, Homo sapiens, Mus musculus, Drosophila melanogaster, Danio rerio, Saccharomyces cerevisiae, Oryza sativa, Arabidopsis thaliana and Escherichia coli.GC content and GC3 s content at the coding region of bovine genome are respectively 0.53±0.09 and 0.60±0.16. It is more affected by base mutation in bovine genome codon usage bias. A handful of genes are affected by the choice. Twenty main preference codons and five high-frequency codons were selected in bovine genome. Correspondence analysis showed that the first axis(Axis1) is to interpret the gene codon preferences reference axis. Axis1 has a strong correlation with GC content, GC3 s content, T3 s content, C3 s content, A3 s content, G3 s content, ENC, CAI and Seqlen, but has a weak correlation with Gravy and Aromo. CAI values were significant negative correlation with ENC value and were significant positive correlation with GC content and GC3 s content. Protein coding genes with the length from 1000 to 2000 bp have high expression level and strong codon preferences. Protein coding genes with the length from 300 to 1000 bp or larger than 2000 bp have low expression level and low codon preferences.All ENC values of mitochondrial genes from bovinae animals were less than 40.50, suggesting that coden usage bias evidently existed. RSCU showed that thirteen codons were prefer preferable in the codons of coding DNA sequences(CDS) of mitochondrial gene. Cluster tree based on the RSCU values of mitochondrial genes from seventeen animal species and phylogenetic tree based on mitochondrial gene sequences are basically consistent. In this study, Bison bison and Bos grunniens were gathered together, Bos javanicus, Bos Taurus, Bos primigenius and Bos indicus were gathered together.In conclusion, yak and bovine genome have codon usage bias, respectively. It was worthy of attention in the study of genome structure and function in yak and bovine genome, as well as adaptability analysis.