Study On The Responses To Salt Stress Of Barley Microspore Mutant In Different Growth Stage

Preliminary identification by salt stress, from the combination of small spores from mutagenesis in vitro culture regenerated plants acquired strains were screened with the original batch of salt tolerance varieties "H30"(equivalent to the wild-type) are different mutants.With mutants as materials, through seedling, tillering and jointing stage of maturity salt stress compared with the H30 mutant morphological characteristics change,in order to find a link with the salt tolerance of morphological characteristics, while the relevant the physiological and biochemical indexes were determined in order to analyze and salt tolerance index link may exist.1.Morphological indicators of performance at different growth stagesThrough the study of seedling was observed at 300 m MNa Cl stress, relative height,the relative number of tillers and relatively dry weight on average, there are differences.From the membership function may reflect small differences in salt tolerance mutant spore material. A7-12 comprehensive salt tolerance is better than the parental H30Through the study of tillering jointing stage, observed that the relative height,relative root length, the relative dry weight and relatively tiller, there are significant differences between the test material variants, from the membership function relationships may reflect the small spore mutant material differences in salt tolerance,A7-20, A7-12 and A7-6 comprehensive salt better than other small spore mutant materials, and higher than the parental H30.Through the study of maturity, observed: A7-20 on four agronomic traits relatively solid grains, relatively effective panicles and relatively strong rate, showed the advantages, and other tested materials that are distinctly different, and A7-20 in the case of a high concentration of salt stress by itself yield has not been much affected2.Physiological and biochemical indicators of performance at different growth stagesBy studying the seedling stage, observed: In the early days of salt stress(0-6h),different test materials POD, SOD and CAT levels were on the rise; mutant CAT activity in different A7-12 and H30 response time point line, the higher the relative content of CAT H30’s. These activity changes and plant salt tolerance may exist some connection;aboveground plant body potassium content and SOD activity showed a significant linear correlation. We speculate that, K + may be involved in regulating the activity of SOD.Shoot two materials K + / Na + ratio there was a significant difference, A7-12 significantly higher than H30.Through the study of tillering jointing stage, observed: small mutant spore material differences in salt tolerance, A7-20, A7-12 and A7-6 comprehensive salt better than other small mutant spore material, and high in parental H30. Aboveground mutant material A7-20 of K+/Na+ highest, and Sk, Na transport under different stress conditions are the highest salt concentration, A7-20 mutant material that has been enhanced salt tolerance.Through the study of maturity, observed: At 300 m M salt stress, K+/Na+ of the varieties are also different, which K+/Na+ of A7-20 is the maximum, much higher than the rest of the mutant materials, and K+/Na+ of parental H30 is minimum. Description A7-20 salt tolerance has been improved.On the basis of the findings can be considered that small spore culture of tolerance can create variants derive tolerant germplasm.The research results provides some scientific clues from the morphological, physiological and biochemical indicators to determine the salt tolerance.And it also provide a scientific basis for small spore viability parsing mechanism of salt tolerance mutant.Characteristics and InnovationDerived from induced mutant microspore culture, its variation caused by mutagenesis, in vitro culture and clone variation.Variation is really abundant and it is easy to occur salt resistance of new mutations.Microspore mutants and the original species(wild type) are similar to near isogenic line.Any one of the variation should be linked with the mutated genes.Microspore mutant is double haploid,so all the alleles are homozygous condition.The character cantroled by homozygous alleles has a high genetic stabilityUnder salt stress, through dentification and comparison of mutants with the original variety of morphological characteristics in the whole stages, it is more easier to discover the physiological and biochemical indicators related to salt tolerance...