Pathogenic Investigation Of Viral Diarrhea Of Piglet Of Liaoning Province And Genetic Variation Of PEDV

Pigs have been found to cause infectious diarrhea virus were mainly porcine epidemic diarrhea virus(PEDV), porcine transmissible gastroenteritis virus(TGEV) and porcine rotavirus(RV), these three pathogen infection and pathogenesis pathways are similarly, the incidence of acute has spread fast, piglets infection and mortality characteristics, clinical diagnostics and control difficult. In recent years, piglets’ infectious viral diarrhea of Liaoning province is serious in, leading tremendous economic losses to the pig industry. In order to identify the major pathogen species of several diarrheas in piglets of Liaoning province, in order to survey the main pathogenic types of viral diarrhea piglets in Liaoning province, develop targeted prevention and control measures; develop targeted prevention and control measures. Carried out the etiology of viral diarrhea piglets’ survey in Liaoning province and genetic variation analysis of PEDV.1. Etiology investigation of piglet diarrhea virusWith the established RT-PCR methods, a total of 335 clinical samples from nine cities of Liaoning were tested for presence of PEDV, TGEV, and RV from April 2013 to next year March. According to the time and place of gathering material disease to statist. The results showed positive for the virus PEDV and TGEV, the positive rate was 38.2% and 15.2%, respectively, both of which were distributed in nine cities. Description Liaoning Province piglets’ viral diarrhea is the leading cause of PEDV, TGEV second; PEDV and TGEV infection are widely distributed in Liaoning Province, the pollution degree is higher, and throughout the year.2. Isolation and identification of Porcine Epidemic Diarrhea LN-J strainClinical investigations have found that the vaccine injection PEDV farms still eventuate, In order to eventuate the source and PEDV pandemic strain variation of Liaoning province. The virus has been isolated and identified, and genetic variation analysis. Acquisition of a suspected PED piglet diarrhea intestinal contents of Liaoning, applications Vero cells were subculture strain isolated strains of the virus by electron microscopy of spherical particles of about 130nm; identified by RT-PCR, the virus is an RNA virus and nucleic acid fragment size with the same primers designed PEDV through nucleic acid sequence analysis proved PEDV, tentatively named PEDVLN-J.3. Porcine epidemic diarrhea analysis of genetic variation of S gene of LN-JBecause of PEDV S gene encoding the surface glycoprotein epitomes’, so the S gene mutation may cause the virus antigens and virulence change, so choose the S gene genetic variation analysis. S designed two pairs of whole genome amplification primers S1 and S2, performed RT-PCR amplification, and successfully obtained fragment sizes correspond bands, and built pUM-S1 and pUM-S2 plasmid, sequenced by bio software for analysis, stitching, to get the whole gene 4161 bp, the use of DNAStar analysis, S gene PEDVLN-J strains, strains and many other places have high homology, phylogenetic and found that the strain HB-2011-1, BJ-2001-2, CHGD-01 closer genetic relationship. Description Liaoning Province is popular PEDV input sexually transmitted, but there is variation in the trend. In the prevention and control process, should consider strengthening the focus on the input and select the appropriate quarantine pig vaccine immunization.