Screen Ning Of Ge Enes Rela Ated To Hi Gh Yield Of Ginsen Nosides

Panax ginseng C.A. Meyer, as a traditional medicine has been utilized in China for a long time.Ginsenosides as the main effective components of ginseng has many kinds of pharmacological activity.The content of the active components are the main factors that influence the quality of medicinal plant.The effective ingredients of medicinal plants are secondary metabolites, while the secondary metabolites in plants often appeared polymorphism and accumulate in specific parts of the plant.Because of the same plant in different populations or individuals the contents of effective ingredients will exist huge difference. And this difference is controlled by genes in a large extent.The contentdeterminationof effective component and molecular marker technology are used to screen genes related to high yield of ginsenosides.The combined use of these two kinds of technology make fast, accurate to evaluate the quality of ginseng possible.In this experiment, the HPLC and SRAP technology were used to screen genes related to high yield of ginsenosides.1.The content of ginsenosides for ginseng in the same region was significantly different.The total ginsenosidesof DH-2 was significantly higher than other sample in Dunhua area. The comparison of monomer ginsenoside showed thatthe content of Rg1, Reand Rfwere significantly higher than other sample in Jian area; the content of Rd,Rb3, Rb2 and Rcwere significantly higher than other sample in Jingyu area.2.The best PCR system of SRAP:DNA2g/L,d NTP 0.39mmol/L, Mg2+ 1.4mmol/L,the forward primerand reverse primer 0.38μmol/μL,Taq enzyme Taq0.02U/μL。3. Five DNA sequences were got by the HPLC and SRAP technologyin this experiment. One was related to the total ginsenosides.Two out of fivewererelated toprotopanaxadiol saponins. And the others were related to Protopanaxatriol saponins.4. In this study, the G1 was successfully got by PCR and expressed in BL21 strain induced by IPTG.Functional verification shows that the protein can effectively improve the content of protopanaxadiol type ginsenosides, and had no significant influenceon synthesis of other types of ginseng saponins.